LOCUS       sequence-146076-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac expression gateway destination vector with 
            Insulator-flanked EF1a promoter gateway cassette and floxed Hygro 
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC150-PBLHL-4xHS-EF1a-DEST
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   118..180
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg
       61 taatacgact cactataggg cgaattgaat ttagcggccg cgaattcgcc cttttaaccc
      121 tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg
      181 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
      241 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
      301 ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttaactag
      361 tatgtcgagc cggcgcgtac tagtggccgg ccagtctgca atgaaatcga gttctgtcat
      421 tctaaatctc tctttcagcc taaagctttt tccccgtatc cccccaggtg tctgcaggct
      481 caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg
      541 gctgtccccg cacgctgccg gctcggggat gcggggggag cgccggaccg gagcggagcc
      601 ccgggcggct cgctgctgcc ccctagcggg ggagggacgt aattacatcc ctgggggctt
      661 tggggggggg ctgtccccgt gagctcccac tagactgtca ttctaaatct ctctttcagc
      721 ctaaagcttt ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg
      781 ttca
//
