LOCUS       sequence-146081-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Toehold switch sensor to detect Zika Virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Pardee K, Green AA, Takahashi MK, Braff D, Lambert G, Lee JW, 
            Ferrante T, Ma D, Donghia N, Fan M, Daringer NM, Bosch I, Dudley DM,
            O'Connor DH, Gehrke L, Collins JJ
  TITLE     Rapid, Low-Cost Detection of Zika Virus Using Programmable 
            Biomolecular Components.
  JOURNAL   Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 
            10.1016/j.cell.2016.04.059.
  PUBMED    27160350
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2016 
            May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059."
COMMENT     Sequence Label: ZIKV_Sensor_32B_LacZ
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     125..144
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        125..143
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             251..424
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
     primer_bind     complement(271..288)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(271..287)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(280..302)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg
       61 gtgtccggga tctcgacgct ctcccttatg gtagtctgcc agtggttcgc gatatagctc
      121 gcgctaatac gactcactat agggctggga tcaagtacat gtagtgcgcc acgagcaaaa
      181 ggactttaga acagaggaga taaagatgtt ttgctcgtgt aacctggcgg cagcgcaaaa
      241 gatgcgtaaa atgaccatga ttacggattc actggccgtc gttttacaac gtcgtgactg
      301 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
      361 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
      421 cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg
      481 cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga
      541 tgcgcccatc tacaccaacg tgacctatcc cattacggtc aatccgccgt ttgttcccac
      601 ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga
      661 aggccagacg cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg
      721 gcgctgggtc ggttacggcc aggacagtcg tttgccgtct gaatttgacc tga
//
