LOCUS       sequence-146158-        4823 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mRFP1 expressed behind an AHL-inducible construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Florea M, Hagemann H, Santosa G, Abbott J, Micklem CN, 
            Spencer-Milnes X, de Arroyo Garcia L, Paschou D, Lazenbatt C, Kong 
            D, Chughtai H, Jensen K, Freemont PS, Kitney R, Reeve B, Ellis T
  TITLE     Engineering control of bacterial cellulose production using a 
            genetic toolkit and a new cellulose-producing strain.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):E3431-40. doi: 
            10.1073/pnas.1522985113. Epub 2016 May 31.
  PUBMED    27247386
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1522985113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-14- 14"; volume: "113"; issue: "24"; pages: 
            "E3431-40"
COMMENT     Sequence Label: pLux02
FEATURES             Location/Qualifiers
     source          1..4823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    26..44
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             63..74
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      878..949
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      965..992
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             1070..1081
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1088..1765
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      1810..1881
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1897..1924
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      1956..2050
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        2098..2200
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2201..2860
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2267..2286)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     oriT            3009..3117
                     /note="incP origin of transfer"
     CDS             complement(3128..3790)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      3791..4562
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     terminator      complement(4677..4763)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4778..4800
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     misc_feature    4802..4823
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagtga aagaggagaa
     1081 atactagatg gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat
     1141 ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta
     1201 cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg
     1261 ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga
     1321 catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa
     1381 cttcgaagac ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt
     1441 catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa
     1501 aaaaaccatg ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa
     1561 aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa
     1621 aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa
     1681 actggacatc acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga
     1741 aggtcgtcac tccaccggtg cttaataacg ctgatagtgc tagtgtagat cgctactaga
     1801 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1861 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1921 tgcgtttata tactagaagc ggccgctgca gcttggactc ctgttgatag atccagtaat
     1981 gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat
     2041 tggtgagaat ccaggggtcc ccaataatta cgatttaaat tggcgaaaat gagacgttga
     2101 tcggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt
     2161 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     2221 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     2281 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     2341 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     2401 gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag
     2461 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     2521 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     2581 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
     2641 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     2701 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     2761 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
     2821 attacaacag tactgcgatg agtggcaggg cggggcgtaa tttgactttt gtccttttcc
     2881 gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc
     2941 gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg
     3001 cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact
     3061 gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccgtag
     3121 gccggcccta ccggcgcggc agcgttaccc gtgtcggcgg ctccaacggc tcgccatcgt
     3181 ccagaaaaca cggctcatcg ggcatcggca ggcgctgctg cccgcgccgt tcccattcct
     3241 ccgtttcggt caaggctggc aggtctggtt ccatgcccgg aatgccgggc tggctgggcg
     3301 gctcctcgcc ggggccggtc ggtagttgct gctcgcccgg atacagggtc gggatgcggc
     3361 gcaggtcgcc atgccccaac agcgattcgt cctggtcgtc gtgatcaacc accacggcgg
     3421 cactgaacac cgacaggcgc aactggtcgc ggggctggcc ccacgccacg cggtcattga
     3481 ccacgtaggc cgacacggtg ccggggccgt tgagcttcac gacggagatc cagcgctcgg
     3541 ccaccaagtc cttgactgcg tattggaccg tccgcaaaga acgtccgatg agcttggaaa
     3601 gtgtcttctg gctgaccacc acggcgttct ggtggcccat ctgcgccacg aggtgatgca
     3661 gcagcattgc cgccgtgggt ttcctcgcaa taagcccggc ccacgcctca tgcgctttgc
     3721 gttccgtttg cacccagtga ccgggcttgt tcttggcttg aatgccgatt tctctggact
     3781 gcgtggccat gcttatctcc atgcggtagg ggtgccgcac ggttgcggca ccatgcgcaa
     3841 tcagctgcaa cttttcggca gcgcgacaac aattatgcgt tgcgtaaaag tggcagtcaa
     3901 ttacagattt tctttaacct acgcaatgag ctattgcggg gggtgccgca atgagctgtt
     3961 gcgtaccccc cttttttaag ttgttgattt ttaagtcttt cgcatttcgc cctatatcta
     4021 gttctttggt gcccaaagaa gggcacccct gcggggttcc cccacgcctt cggcgcggct
     4081 ccccctccgg caaaaagtgg cccctccggg gcttgttgat cgactgcgcg gccttcggcc
     4141 ttgcccaagg tggcgctgcc cccttggaac ccccgcactc gccgccgtga ggctcggggg
     4201 gcaggcgggc gggcttcgcc cttcgactgc ccccactcgc ataggcttgg gtcgttccag
     4261 gcgcgtcaag gccaagccgc tgcgcggtcg ctgcgcgagc cttgacccgc cttccacttg
     4321 gtgtccaacc ggcaagcgaa gcgcgcaggc cgcaggccgg aggcttttcc ccagagaaaa
     4381 ttaaaaaaat tgatggggca aggccgcagg ccgcgcagtt ggagccggtg ggtatgtggt
     4441 cgaaggctgg gtagccggtg ggcaatccct gtggtcaagc tcgtgggcag gcgcagcctg
     4501 tccatcagct tgtccagcag ggttgtccac gggccgagcg aagcgagcca gccggtggcc
     4561 gctcgcggcc atcgtccaca tatccacggg ctggcaaggg agcgcagcga ccgcgcaggg
     4621 cgaagcccgg agagcaagcc cgtagggggg gcgcgcccag ctgtctaggg cggcggattt
     4681 gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc
     4741 gactgagcct ttcgttttat ttgatgcctt taattaaagc ggataacaat ttcacacagg
     4801 agaattcgcg gccgcttcta gag
//
