LOCUS       sequence-146176-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of Grap2 R83E in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Chougule RA, Cordero E, Moharram SA, Pietras K, Ronnstrand L, Kazi 
            JU
  TITLE     Expression of GADS enhances FLT3-induced mitogenic signaling.
  JOURNAL   Oncotarget. 2016 Feb 15. doi: 10.18632/oncotarget.7415.
  PUBMED    26895103
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncotarget.
            2016 Feb 15. doi: 10.18632/oncotarget.7415."
COMMENT     Sequence Label: pcFLAG-Grap2-R83E
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..31)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             483..494
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 attcttactt gtcatcgtca tccttgtagt cctcgagtcg ggtcatgggt gccacgtagt
       61 tggcagggaa gaggcccagc ttgttgtgca ggcggccggt ccaccaggat gggttggagc
      121 tatccaggac ctccaccacc tccccgctgt ggaaccccag ctcgtcatcc tccagggcct
      181 caaagtcata cagcgcccgg gcccaccgca ctcgccctgc cgcctggagc tgcactgggt
      241 ctgtgtgtct ccgatgcatg agggccgcat tcatttcact gcccaagccg gtgccacaat
      301 gcccatcatt tatgtcaagg ctgcctcctc ggcgttcctg gtggaaatgg tggtgctgca
      361 gatatcgctg ctgtgggggc tgctgcagct gctggggcgc tggggcatat tgcggaggct
      421 gtggctggtg ctggtgctgc tgcaggggaa gggtcggggg gtgatccgac agcttccggt
      481 tcatcgaagg tcggatttct tctcccacag ccccactgag gtgtgggcct ccctgggacc
      541 tccggtccag gctgttgccc cggtgaccct ggtcttctcg ggttctgtct ctaaggaaga
      601 tctgcttctg tctggagatg gaatttgtcc tgtagtagtc taccagctta tttagggatg
      661 gaaacttctc agtccacaga aagtaattac ccttgttgtc tcgcatgacc ttgaagtgtt
      721 gaacgtcatc ctcatgcctg acagagatgg agaagtccc
//
