LOCUS       sequence-146177-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of Grap2 R83E in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Chougule RA, Cordero E, Moharram SA, Pietras K, Ronnstrand L, Kazi 
            JU
  TITLE     Expression of GADS enhances FLT3-induced mitogenic signaling.
  JOURNAL   Oncotarget. 2016 Feb 15. doi: 10.18632/oncotarget.7415.
  PUBMED    26895103
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncotarget.
            2016 Feb 15. doi: 10.18632/oncotarget.7415."
COMMENT     Sequence Label: pcFLAG-Grap2-R83E
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(645..656)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
       61 ctagcgttta aacttaagct tggtaccgag ctcggatcgg ccaccatggg atccgaagct
      121 gttgccaagt ttgatttcac tgcttcaggt gaggatgaac tgagctttca cactggagat
      181 gttttgaaga ttttaagtaa ccaagaggag tggtttaagg cggagcttgg gagccaggaa
      241 ggatatgtgc ccaagaattt catagacatc cagtttccca aatggtttca cgaaggcctc
      301 tctcgacacc aggcagagaa cttactcatg ggcaaggagg ttggcttctt catcatcgag
      361 gccagccaga gctccccagg ggacttctcc atctctgtca ggcatgagga tgacgttcaa
      421 cacttcaagg tcatgcgaga caacaagggt aattactttc tgtggactga gaagtttcca
      481 tccctaaata agctggtaga ctactacagg acaaattcca tctccagaca gaagcagatc
      541 ttccttagag acagaacccg agaagaccag ggtcaccggg gcaacagcct ggaccggagg
      601 tcccagggag gcccacacct cagtggggct gtgggagaag aaatccgacc ttcgatgaac
      661 cggaagctgt cggatcaccc cccgaccctt cccctgcagc agcaccagca ccagccacag
      721 cctccgcaat atgccccagc gccccagcag ctgcagcagc ccccacagca gcgatatctg
      781 cagcaccacc atttccacca gga
//
