LOCUS       sequence-146184-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector containing flag tagged S155,172E MFF 
            isoform 5 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Toyama EQ, Herzig S, Courchet J, Lewis TL Jr, Loson OC, Hellberg K, 
            Young NP, Chen H, Polleux F, Chan DC, Shaw RJ
  TITLE     Metabolism. AMP-activated protein kinase mediates mitochondrial 
            fission in response to energy stress.
  JOURNAL   Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
  PUBMED    26816379
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-01-15- 15"; volume: "351"; 
            issue: "6270"; pages: "275-81"
COMMENT     Sequence Label: pLenti.PGK.blast-Flag-MFF-S155,172E
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    55..79
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(807..830)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgag cggccgccac tgtgctggat atcaaccact
       61 ttgtacaaga aagctgggtc ctagcggcga aaccagagcc agctattaag cagccagaaa
      121 gctacagtaa ttgaatacat gaccatttct cttttagcac gttctttgtt ctcctcttcc
      181 agaagttgta gacgtctatt tagtttgatt atctgtcgtc ttagtgaagc tgcatctaca
      241 acagtcaggt catctgacgt tccttcaatg gttgtatcta tatttgaaat gccatacctg
      301 acgttgtcat gatgaggatt agaagtggcg gcagcagacc caccacgcaa cacaggtagt
      361 tcatcatttc tgaccagctg tccattttgg cgaacagcat tttcactcat ctcccgctct
      421 ctttttagtc tgccaactgc tcggatttct tcattttgag gggttgtagg aggtctttct
      481 aaatccagaa aatctagtgg tctttcactc agcgtaagta cacgaggtgg tgttttcagt
      541 gccaggggtt taaagggagt tgactgaata aggtcaagat ctgctggtct tgaaaatgaa
      601 acatcttcat tatttcctgc tacaacaatc ctctccggaa cttgcattat cacactagca
      661 tttggaactc cttcttggaa tccttgttcc aggtcagcgt ttggcggtgc tacttttaac
      721 ttttctggga ccctcattcg ctgactaatg ccttcagtat attccatttc gtactgaatt
      781 cgactaattt ctgccataga tccgcccttg tcatcgtcgt ccttgtaatc catgg
//
