LOCUS       sequence-146193-       11454 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transfer vector encoding Mars1Cy-GFP membrane-spanning 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11454)
  AUTHORS   Zhang M, Chakraborty SK, Sampath P, Rojas JJ, Hou W, Saurabh S, 
            Thorne SH, Bruchez MP, Waggoner AS
  TITLE     Fluoromodule-based reporter/probes designed for in vivo fluorescence
            imaging.
  JOURNAL   J Clin Invest. 2015 Oct 1;125(10):3915-27. doi: 10.1172/JCI81086. 
            Epub 2015 Sep 8.
  PUBMED    26348895
REFERENCE   2  (bases 1 to 11454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1172/JCI81086"; 
            journalName: "J Clin Invest"; date: "2015-10-1- 1"; volume: "125"; 
            issue: "10"; pages: "3915-27"
COMMENT     Sequence Label: pLenCMV-PMMars1CyGFP
FEATURES             Location/Qualifiers
     source          1..11454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2662..2965
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2966..3169
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3208..3217
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     3214..3273
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             3277..3303
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(4162..4164,4165..4167,4168..4881)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4162..4164,4165..4167,4168..4878)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4207..4228)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4468..4487)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4815..4836
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(4914..5487,5488..5490,5491..5499)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5081..5098)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5308..5327
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5519..6118
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(5519..5538)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6015..6035
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6146..6734
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6199..6219)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6617..6628)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6737..6753)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6738..6754)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7259..7439
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7465..7482)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7471..7695
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7741..8169
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7828..7847)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8038..8059
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8178..8198)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8183..8512
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8363..8498
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8425..8444
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8560..8607
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8626..9000
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9130..9251
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9167..9186)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9221..9240
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9300..9316)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9300..9316)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9313..9335)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9324..9340
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9348..9354,9355..9372,9373..9378))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9393..9414
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9531..9548)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9702..10290)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9782..9801)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10461..11252,11253..11321))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11084..11103
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11322..11426)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta atagttatta atagtaatca attacggggt cattagttca
     2641 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     2701 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     2761 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     2821 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     2881 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     2941 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     3001 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     3061 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     3121 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     3181 ccgtcagatc cgctagcgct accggtcgcc accatggaga cagacacact cctgctatgg
     3241 gtactgctgc tctgggttcc aggttccact ggtgactatc catatgatgt tccagattat
     3301 gcttctagct cttacgaatt aacacaacct ccatctgttt ctgtttctcc aggtcaaact
     3361 gctagaatta cttgttctgg tgatgctttg ccaaaacaat acacttactg gtatcaacaa
     3421 aaagctggtc aagctccagt tttggttatt tataaagata ctgaaagacc atctggtatt
     3481 ccagaaagat tctcaggtac ttcttctggt actactgcta ctttgactat ttctggtgtt
     3541 caagctgaag atgaagctga ttattattgt caatctgctg attcttctgg ttctcatgta
     3601 tttttcggtg gtggtactaa agttactgtt ttgtctggtg gaggaggccc aggtggcggt
     3661 tcaggtggag gttctggcgg aggttctggt ggaggttcct atgagttgac tcagccaccc
     3721 tcggtgtcag tgtccccagg acagacggcc aggatcacct gctctggaga tgcattgcca
     3781 aagcaatata cttattggta ccagcagaag gcaggccagg cccctgtctt ggtgatatat
     3841 aaagacactg agaggccctc agggatccct gagcgattct ctggtaccag ttcagggaca
     3901 acagccacat tgaccatcag tggagtccag gcagaagacg aggctgacta ttactgtcaa
     3961 tcagcagaca gcagtggttc ccacgttttc ttcggcggag ggaccaaggt gaccgtacta
     4021 tccggaggcg gtgggaagaa gttcccccca gaagaccctc ctgatagcaa gaacacactt
     4081 gtgctctttg gggcaggatt cggcgcagta ataacagtcg tcgtcatcgt tgtcatcatc
     4141 aaatgcttct gtaagcacag aatggtgagc aagggcgagg agctgttcac cggggtggtg
     4201 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     4261 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     4321 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     4381 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     4441 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     4501 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     4561 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     4621 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     4681 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     4741 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     4801 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     4861 atggacgagc tgtataagta atgtacatag taattaacgc atccggattt ccgcccctct
     4921 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     4981 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     5041 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     5101 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     5161 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     5221 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     5281 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     5341 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     5401 tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     5461 ttttcctttg aaaaacacga tgataagctt gccacaaccc acaaggagac gaccttccat
     5521 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     5581 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     5641 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     5701 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     5761 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     5821 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     5881 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     5941 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     6001 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     6061 cgtcgagtgc ccgaaggacc gcgcgacctg gtgcatgacc cgcaagcccg gtgcctgata
     6121 ggaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     6181 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6241 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6301 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6361 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6421 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6481 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6541 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     6601 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6661 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     6721 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac
     6781 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     6841 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     6901 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     6961 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     7021 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
     7081 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     7141 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     7201 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     7261 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7321 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7381 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7441 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     7501 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     7561 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     7621 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     7681 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     7741 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7801 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7861 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7921 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7981 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     8041 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     8101 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     8161 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     8221 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     8281 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     8341 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8401 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     8461 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     8521 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     8581 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     8641 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     8701 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     8761 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     8821 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     8881 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     8941 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     9001 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     9061 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     9121 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9181 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9241 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     9301 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9361 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     9421 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9481 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9541 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     9601 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     9661 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     9721 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     9781 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     9841 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     9901 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     9961 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    10021 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    10081 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    10141 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    10201 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    10261 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    10321 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    10381 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    10441 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    10501 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    10561 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    10621 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    10681 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    10741 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    10801 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    10861 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    10921 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    10981 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    11041 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    11101 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    11161 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    11221 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    11281 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    11341 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    11401 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
