LOCUS       sequence-146198-        3827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Cux1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Cux1
FEATURES             Location/Qualifiers
     source          1..3827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(952..970)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(953..970)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(988..1004)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(988..1004)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1001..1023)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1012..1028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1036..1042,1043..1060,1061..1066))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1081..1102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1219..1236)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1390..1978)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1470..1489)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2149..2940,2941..3009))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2772..2791
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3010..3114)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3192..3647)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3329..3350)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3541..3560
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3773..3795
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3787..3804
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3788..3804
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3811..3827,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3811..3827,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tctcagaaag cactccaaag acctctgcca gctgcagccc tgcccctgag tccccaatga
      121 gttccagcga atctgtgaag agtctcaccg agctggtcca gcagccctgt cccgccattg
      181 agaccagtaa agaaggcaaa ccaccagagc ccagcgaccc gcctgcctca gactcccagc
      241 ccacaacacc gctgcctctc tctggacact cagccctcag catccaagaa ttagtagcca
      301 tgtctcctga gctggacacg tatggcataa ccaagagggt caaagaggtg ctgacagaca
      361 acaacctcgg tcagcgctta tttggggaga ccattttggg gctcacccag ggttctgtct
      421 ccgacctcct tgcccgccca aagccttggc ataagcttag tctgaaagga cgggaaccct
      481 ttgtccgcat gcagctatgg ctgaacgacc ccaacaatgt ggagaagctg atggatatga
      541 agcggatgga gaagaaagcc tacatgaaga ggcgacacag ctctgtcagt gacagtcagc
      601 cttgtgagcc cccctctgtg ggtattgact atagccaagg cgccagcccc cagccacaac
      661 accagctgaa gaaacctcga gtggtgctgg ctccagagga gaaggaagcg ctgaagcgag
      721 catatcagca gaagccatac ccatcaccaa aaaccatcga ggaacttgcc acacaactca
      781 acctgaagac cagcaccgtc atcaactggt tccataatta caggtctcgg atccgcagag
      841 aactgttcat tgaggagatt caagctggaa gaatcactag tgaattcgcg gccgcctgca
      901 ggtcgaccat atgggagagc tcccaacgcg ttggatgcat agcttgagta ttctatagtg
      961 tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     1021 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     1081 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     1141 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     1201 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     1261 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     1321 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1381 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1441 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1501 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1561 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1621 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1681 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1741 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1801 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1861 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1921 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1981 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     2041 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     2101 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     2161 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2221 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     2281 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     2341 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     2401 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2461 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2521 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2581 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2641 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2701 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     2761 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2821 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2881 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     2941 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     3001 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3061 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     3121 tccccgaaaa gtgccacctg atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     3181 accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     3241 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     3301 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     3361 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     3421 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     3481 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     3541 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     3601 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc
     3661 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     3721 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     3781 cgacgttgta aaacgacggc cagtgaattg taatacgact cactata
//
