LOCUS       sequence-146202-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Mates L, Chuah MK, Belay E, Jerchow B, Manoj N, Acosta-Sanchez A, 
            Grzela DP, Schmitt A, Becker K, Matrai J, Ma L, Samara-Kuko E, 
            Gysemans C, Pryputniewicz D, Miskey C, Fletcher B, VandenDriessche 
            T, Ivics Z, Izsvak Z
  TITLE     Molecular evolution of a novel hyperactive Sleeping Beauty 
            transposase enables robust stable gene transfer in vertebrates.
  JOURNAL   Nat Genet. 2009 Jun;41(6):753-61. Epub 2009 May 3.
  PUBMED    19412179
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Genet."; date: "2009-06"; volume: "41(6)"; pages: "753-61. Epub 2009
            May 3"
COMMENT     Sequence Label: pCMV(CAT)T7-SB100
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    98..232
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(135..154)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     189..208
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 agcttgcatg cctgcaggtc gactctagag gatccgaaaa aacctcccac acctccccct
       61 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
      121 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
      181 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccccgcggt
      241 tagggcccct agtatttggt agcattgcct ttaaattgtt taacttgggt caaacgtttc
      301 gggtagcctt ccacaagctt cccacaataa tttgggtgaa ttttggccca ttcctcctga
      361 cagagctggt gtaactgagt caggtttgta ggcctccttg ctcgcacacg ctttttcagt
      421 tctgcccaca aattttctat aggattgagg tcagggcttt gtgatggcca ctccaatacc
      481 ttgactttgt tgtccttaag ccattttgcc acaactttgg aagtatgctt ggggtcattg
      541 tcgtgttgga agacccattt gcgaccaagc tttaacttcc tgactgatgt cttgagatgt
      601 tgcttcaata tatccacata ctgcaccgcg tccatgatgc catctatttt gtgaagtgca
      661 ccagtccctc ctgcagcaaa gcacccccac aacatgatgc tgccaccccc gtgcttcacg
      721 gttgggatgg tgttcttcgg cttgcaagcc tcccccttct tcctccaaac ataacgatgg
      781 tcattatggc caaacagttc tatttttgtt tcatcagacc agaggacatt tctccaaaaa
      841 gtacgatctt tgtccccatg tgcagttgca a
//
