LOCUS       sequence-146203-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Mates L, Chuah MK, Belay E, Jerchow B, Manoj N, Acosta-Sanchez A, 
            Grzela DP, Schmitt A, Becker K, Matrai J, Ma L, Samara-Kuko E, 
            Gysemans C, Pryputniewicz D, Miskey C, Fletcher B, VandenDriessche 
            T, Ivics Z, Izsvak Z
  TITLE     Molecular evolution of a novel hyperactive Sleeping Beauty 
            transposase enables robust stable gene transfer in vertebrates.
  JOURNAL   Nat Genet. 2009 Jun;41(6):753-61. Epub 2009 May 3.
  PUBMED    19412179
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Genet."; date: "2009-06"; volume: "41(6)"; pages: "753-61. Epub 2009
            May 3"
COMMENT     Sequence Label: pCMV(CAT)T7-SB100
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          128..224
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     281..300
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        281..299
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
       61 accgatccag cctccggact ctagaggatc cggtactcga ggaactgaaa aaccagaaag
      121 ttaactggta agtttagtct ttttgtcttt tatttcaggt cccggatccg gtggtggtgc
      181 aaatcaaaga actgctcctc agtggatgtt gcctttactt ctaggcctgt acggaagtgt
      241 tacttctgct ctaaaagctg cggaattgta cccgcggaat taatacgact cactataggg
      301 actagtacca tgggaaaatc aaaagaaatc agccaagacc tcagaaaaag aattgtagac
      361 ctccacaagt ctggttcatc cttgggagca atttccaaac gcctggcggt accacgttca
      421 tctgtacaaa caatagtacg caagtataaa caccatggga ccacgcagcc gtcataccgc
      481 tcaggaagga gacgcgttct gtctcctaga gatgaacgta ctttggtgcg aaaagtgcaa
      541 atcaatccca gaacaacagc aaaggacctt gtgaagatgc tggaggaaac aggtacaaaa
      601 gtatctatat ccacagtaaa acgagtccta tatcgacata acctgaaagg ccactcagca
      661 aggaagaagc cactgctcca aaaccgacat aagaaagcca gactacggtt tgcaactgca
      721 catggggaca aagatcgtac tttttggaga aatgtcctct ggtctgatga aacaaaaata
      781 gaactgtttg gccataatga ccatcgttat gtttggagga agaaggggga ggcttgcaag
      841 ccgaagaaca ccatcccaac cgtgaagcac gggggtggca gcatcatgtt gtggggg
//
