LOCUS       sequence-146211-         651 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express C-terminal YC fused influenza A virus partial NS1 protein 
            (27-236 nt) for dsRNA labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Cheng X, Deng P, Cui H, Wang A
  TITLE     Visualizing double-stranded RNA distribution and dynamics in living 
            cells by dsRNA binding-dependent fluorescence complementation.
  JOURNAL   Virology. 2015 Nov;485:439-51. doi: 10.1016/j.virol.2015.08.023. 
            Epub 2015 Sep 7.
  PUBMED    26351203
REFERENCE   2  (bases 1 to 651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2015-11"; volume: "485:439-51. doi"; pages: " 
            10.1016/j.virol.2015.08.023. Epub 2015 Sep 7"
COMMENT     Sequence Label: pdRBFC-NS1-YC
FEATURES             Location/Qualifiers
     source          1..651
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(88..109)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    296..320
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(537..561)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(565..588)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    593..647
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
ORIGIN
        1 tctctagact cacctagaat taactctcta gactcaccta ggtcacttgt acagctcgtc
       61 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
      121 ctcgttgggg tctttgctca gggcggactg gtagctcagg tagtggttgt cgggcagcag
      181 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc
      241 gtggttcatg actttctgct tgaggtcgtt agggatctta caagcaggcc taggcaccac
      301 tttgtacaag aaagctgggt ctttcagaat ccgctccact atctgctttc cagcacgtgt
      361 ggctgtctcg atgtccagac cgagggtgct gccccttcct cttagggatt tctgatctcg
      421 gcgaagccga tcaaggaatg gggcatcacc tagttcttgg tctgcaactc gtttgcggac
      481 atgccaaaga aagcaatcta cctgaaagct tgacacagtg tttggatcca tcatgaagcc
      541 tgcttttttg tacaaacttg tgattttgtc gtcatcgtct ttgtagtcca tggtaattgt
      601 aaatgtaatt gtaatgttgt ttgttgtttg ttgttgttgg taattgttgt a
//
