LOCUS       sequence-146213-         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express C-terminal YN fused Marburg virus partial VP35 protein 
            (3541-3930 nt) for dsRNA labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Cheng X, Deng P, Cui H, Wang A
  TITLE     Visualizing double-stranded RNA distribution and dynamics in living 
            cells by dsRNA binding-dependent fluorescence complementation.
  JOURNAL   Virology. 2015 Nov;485:439-51. doi: 10.1016/j.virol.2015.08.023. 
            Epub 2015 Sep 7.
  PUBMED    26351203
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2015-11"; volume: "485:439-51. doi"; pages: " 
            10.1016/j.virol.2015.08.023. Epub 2015 Sep 7"
COMMENT     Sequence Label: pdRBFC-VP35-YN
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(125..637,638..640,641..643))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     318..337
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     577..598
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    663..687
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atatcatgcg atcataggcg tctcgcatat ctcattaaag caggactcta gggactagtc
       61 ccgggtctta attaactctc tagactcacc tagaattaac tctctagact cacctaggtc
      121 agtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg
      181 ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc
      241 cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga
      301 acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct
      361 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
      421 agcgggcgaa gcactgcagg ccgtagccga aggtggtcac gagggtgggc cagggcacgg
      481 gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct
      541 cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
      601 tgggcaccac cccggtgaac agctcctcgc ccttgctcac cattgtagcg atactcctag
      661 gcaccacttt gtacaagaaa gctgggtcga ttttcagggc ccgtgtctca ccttgctcag
      721 atgaataa
//
