LOCUS       sequence-146216-         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  reprogrammed sortase A variant that recognizes alternative substrate
            LAXTG with high activity and specificity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Dorr BM, Ham HO, An C, Chaikof EL, Liu DR
  TITLE     Reprogramming the specificity of sortase enzymes.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13343-8. doi: 
            10.1073/pnas.1411179111. Epub 2014 Sep 3.
  PUBMED    25187567
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1411179111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-09-16- 16"; volume: "111"; issue: "37"; pages: "13343-8"
COMMENT     Sequence Label: eSrtA(2A-9) pET29b
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             490..512
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    527..551
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(551..570)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(552..570)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(658..677)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgggatcctt tcacttcggt cgccacaaaa
       61 attttacggg attcccacac gccggtttct tcgttataat catcgcaggt caccagggtc
      121 aactgtttat ctttgccttc ctgttcatcc agcacttcca ccgcgttcgg atgcacctta
      181 cgaatgctgg tcattttata tatacgggtt tcgttgccca ctttaaaata caccatgctg
      241 ccaggtttcg ccgctttcag gttggtaaac tgatagttcg gacgatcaat aaaggtatgg
      301 cccgcaatgc taatgttctg atcatccagg ctttcgtttt cgtcatgaaa gcacacgccc
      361 cggttcagct gttcacgggt cgccgggccc ggatacaccg gttctttaat atccgcatcc
      421 ggaatttcaa tataacccgc cactttgctt ttatctttcg gaatctgcgg tttcgcctgc
      481 atatgtatat ctccttctta aagttaaaca aaattatttc tagaggggaa ttgttatccg
      541 ctcacaattc ccctatagtg agtcgtatta atttcgcggg atcgagatcg atctcgatcc
      601 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
      661 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
      721 gtttcggcgt gggtatggtg gcaggccccg tggccgg
//
