LOCUS       sequence-146226-        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of cytosolic APEX2 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, 
            Ting AY
  TITLE     Directed evolution of APEX2 for electron microscopy and proximity 
            labeling.
  JOURNAL   Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
  PUBMED    25419960
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179."
COMMENT     Sequence Label: pTRC-APEX2
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     943..962
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga
       61 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt
      121 ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat
      181 atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg
      241 atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg
      301 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt
      361 gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg
      421 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg
      481 cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag
      541 aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag
      601 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt
      661 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc
      721 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt
      781 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat
      841 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct
      901 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc
      961 gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca
     1021 tgccataccg cgaaaggttt tgcaccattc ga
//
