LOCUS       sequence-146235-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Hook domain of Hook3 in bacteria for purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Schroeder CM, Vale RD
  TITLE     Assembly and activation of dynein-dynactin by the cargo adaptor 
            protein Hook3.
  JOURNAL   J Cell Biol. 2016 Aug 1;214(3):309-18. doi: 10.1083/jcb.201604002.
  PUBMED    27482052
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2016-08-1- 1"; volume: "214"; 
            issue: "3"; pages: "309-18"
COMMENT     Sequence Label: pET28a-Hook3 aa 1-160
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(566..586)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(633..653)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgctta tttactcatc agctcttgaa tggctgtcat gacaacatgt tgaacagatt
      121 cctccatcat cataatggct tggatgtact cttgcttctg ttcacagttc acagcacagc
      181 ctaagatgag ctgaagcatc cttccaagct ctgctgcatc agaatgctcc ccaataaggt
      241 tcacatcagg aagggtaaag tcattaattt gctgtcctaa aatctcatga ttataatcca
      301 agattccttt taaaattttc tttaaattgc ttatctttag cctccaatta tctcctactt
      361 cagttttgat tctgtttagc caattttcat caaaatatgc aggatctatc ttttgaagaa
      421 cctgggccat cacaacccca ttcgttaaat cttccacggt ctggcatggt gcatccacat
      481 taaatgtctg gatccaagtg aggaggctct cgcacagctc cgcccgctcc agcgactcta
      541 cgctgaacat ggtaccacct ccacccccct gaaaatacag attttcggat cctttgtaga
      601 gctcatccat gccatgtgta atcccagcag cagttacaaa ctcaagaagg accatgtggt
      661 cacgcttttc gttgggatct ttcgaaagga cagattgtgt cgacaggtaa tggttgtctg
      721 gtaaaaggac agggccatcg ccaattggag tattttgttg ataatggtct gctagttgaa
      781 cggaaccatc ttcaacgttg tggcgaattt tgaagttagc tttgattcca ttcttttgtt
      841 tgtctgccgt gatgtataca ttgtgtgagt taaagttgta ttcgagtttg tgtccaagaa
      901 tgtttccatc ttctttaaaa tcaataccct ttaactcgat acgat
//
