LOCUS       sequence-146291-       16146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pKT230 plasmid engineered with the RTCas1v2-GFP cassette (RT-Cas1, 
            Cas2, GFP) driven by the MMB-1 16S rRNA promoter, the CRISPR03 array
            driven by its native leader, and the tmRNA::td intron construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16146)
  AUTHORS   Silas S, Mohr G, Sidote DJ, Markham LM, Sanchez-Amat A, Bhaya D, 
            Lambowitz AM, Fire AZ
  TITLE     Direct CRISPR spacer acquisition from RNA by a natural reverse 
            transcriptase-Cas1 fusion protein.
  JOURNAL   Science. 2016 Feb 26;351(6276):aad4234. doi: 
            10.1126/science.aad4234.
  PUBMED    26917774
REFERENCE   2  (bases 1 to 16146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-02-26"; volume: "351(6276):aad4234. doi"; pages: " 
            10.1126/science.aad4234"
COMMENT     Sequence Label: pV92.2
FEATURES             Location/Qualifiers
     source          1..16146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3708..4424
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(3738..3766)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4361..4381
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     rep_origin      6105..6499
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            6841..6928
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             8167..9138
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             9649..10488
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             10475..11326
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     rep_origin      12011..12556
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     12457..12476
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12673..12690
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(13140..13159)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             13151..13966
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIADALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(13224..13243)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        14822..14926
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(16119..16138)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
       61 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
      121 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
      181 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
      241 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
      301 gagcttgccg ccgccgcagg tggagctgac ttcatccgca cacacgagcc gcgccccttg
      361 cgcgacgggc tggcggtatt ggcggcgctg aaagaaaccg caagaattcg ttaactgaac
      421 acgtcaattc gctttacggt ctttcagctt aggttggaag acaaatagtt aaagtcagac
      481 gttattcatg agttagagca aattctttta actgattttt tgggctaaca ggaggaatta
      541 accatgctga attcccctct tatagacgcc gttttgcctt tacgcagtgt ggtcatcaca
      601 ctgcgttggc tgtcgccaag caaaacgggc tttttgcatc atgcgggttt gcacgcttgg
      661 gtacgctttc tcgctggcag tccagaacag tttagcgact ttatcgtcgt cgaacctata
      721 gaaaacggcc acattagtta ccaagcagga gacggttaca ggtttcgaat taccgtgtta
      781 aacggtggtg aaagcctgct cgatacgctc ttctcttcgt taaaacggct cccagaaagc
      841 gccgctaatc acccagacat agcgggcgct ttctcggaca acctcgtctt agaaaaaatc
      901 gaagatacat ttgagcacca tcaagtcaca caaatagaag acctaagcgt gtttgatatt
      961 aatgcgctaa tgctcgaaac cgcggtttgg tcacgtcaac gacggttcaa agtggccttc
     1021 aacaccccag cgaggctcgt caaacccaag ccagaagatg gcacagagct aaaaggccaa
     1081 aaccgttact gccgtgataa atccgactta aactggcagc tctttacgca tcgcctcacc
     1141 gataccttta ttaacctctt tcaaagccga actggcgagc gtttacaaag gcaaaactgg
     1201 cccgaagcgc aactccacgc agggttagcg gtttggctaa acaacagtta caccaataaa
     1261 aaagagaaaa aagtcaaaga cgccagcggc atgttggcgc aaatgcaaat cgagattgat
     1321 gacgacttcc ctgccgattt actcgcgctt cttgttctgg gtcaatacat cggcatgggc
     1381 caaaatcgcg cattcggtat ggggcaatat caactgcaag atgcttatgg ttactgcagc
     1441 taccctcgcc ctcaagcggc caaaagtctg ctcgaaaaaa gtctatctga tgcaagctta
     1501 caccaagcct gccaaaccat gtacccaaga caagctaact ttgactcgtc ggacaccgac
     1561 gaagagcatc acgacgccat cgacgagctg cttaccaaac tctatgtaag ccgcgaacgt
     1621 attttcaaac gagagtttac tccaagccag ttacactctg tcgaaatcga aaagccagaa
     1681 ggcggaacac gactgctgtc ggttcccaat tggcacgacc gcaccttaca aaaagcggta
     1741 acagaatgcc ttggcaacac cctagaacac atatggatga agcacagcta tggctaccga
     1801 aagggacact ctcgcctaca ggcccgagac caaatcaacc aatacattca gcaaggctac
     1861 gaatgggtac tcgaaagcga catagaaagc ttcttcgaca gtgtcaattg gctgaaccta
     1921 gaacaacgac tgaaactact attgcctaat gaaccgttag tccccttact catgcaatgg
     1981 gtcagtgcag cgaaacaaac cgaagatgag caaacgcttg cgcgccataa cggcctgcca
     2041 caaggcgcgc cgatcagtcc gatactggct aacctattac ttgatgacct cgaccaagat
     2101 atgatcgcca aagggcatca aatcgtccgc tacgctgatg attttgtctt actttttaaa
     2161 tcaaaagcgg cggcagaaag cgcactcgac gacataataa cagcccttaa agagcatcat
     2221 ctcgctatca accttgaaaa aacgcgtatt gtcgaagcgt ctcaagggtt ccgctattta
     2281 ggctacttat ttgtcgatgg ctacgcgatc gaaaccaaac gcgaataccg caaagagcac
     2341 gctcaactcg acaaacagct gaacgcctct tcgctcgaaa acgagccctc tctacaacag
     2401 gaacctgctg tacaaaacga acaaagcacg cttattggag agcgcgaaaa gctaggcaca
     2461 ttgctcatca tagcagggga cattgcgatg ctaagcagtg aaaaacaacg cctaatagtc
     2521 gaacaatacg acgagctgca tacttaccct tgggccacgt tgagttccgt gttgcttgta
     2581 ggcccgcacc acatcaccac ccccgctcta aaatcggcta tgttccacaa tgtacccgta
     2641 cactttgcta gccaatatgg ccgctaccaa ggggtttccg caggtgctgc gcccagtgtg
     2701 tttggcgccg atttttggtt acttcaagct caatacttac aacaagaaac aaacgcactc
     2761 aacattagcc aagtgctaat acaagccaga atagagggta taagagccgt cataagccgt
     2821 agagaaaaag atgcccctga gctgaacaaa attcaacgct tggacgaaaa acgcttacgg
     2881 gcagaaacat tagatcaatt acgaggctat gaaggccaag ccagcaaaca actgtgggca
     2941 ttcttccagc gtatactcga agaagattgg ggctttacag ggcgtaatag acgccctccc
     3001 aaagacccga tcaacgccct actttcatta ggctacacct atctatacag cttagtagac
     3061 agtgtcaata gaacggttgg actctaccct tggcaaggcg cactgcacca gcgtcatggt
     3121 taccatcaca ccctagcttc tgacctaatg gaaccttggc gttaccttgt cgaacacgtc
     3181 gtcttaaccc taataaatcg tcaccaaata cataaggatg actttgtcat caaagaaaat
     3241 ggttgcgaaa tgagcagtgg cgcgagaaaa acacttctaa aagaactctt ggttcaactg
     3301 actaaagtgc ctaagggagg aaacagtctg ctcaccgaaa tgagcaacca atcttaccgt
     3361 ttagccctaa gctgcaaaat gcaacaacgc tttatcgctt ggagccctaa aagatgagaa
     3421 tataccttgc ctgtttcgac atcgaagacg ataaaaaacg ccgtaagctc tcaaatttac
     3481 tcttagaata tggcgatcga gtccagtact cggtattcga aatcagccta aaagatgaaa
     3541 acgagctgca taagctgcgt aaaaaatgca gcaaatacac agaagaagca gatagcctgc
     3601 gcttctattg gttaaacaaa gaatccagaa aacacagcca agacgtttgg ggaaacccca
     3661 ttgcggtgtt ccccgccgcc gtcctgttat gacaggagga attaaccatg agtaaaggag
     3721 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
     3781 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
     3841 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttct
     3901 cttatggtgt tcaatgcttt tcaagatacc cagatcatat gaaacggcat gactttttca
     3961 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
     4021 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
     4081 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
     4141 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
     4201 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
     4261 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
     4321 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
     4381 cagctgctgg gattacacat ggcatggatg aactatacaa ataggtttga gctctaaacc
     4441 ctttatcagt gaataaacga tttttgctct ttaaaaacat aaccttaaaa cagtcctcaa
     4501 ttgattgaag gggtttaggg cgcgttttac ataaaaatca aaaacttagc ttgaaataat
     4561 ggcgaaaatt cactaatttt aagcatacct cttgtggata acttgagggc gggggaaacg
     4621 ctaggttaac ctgctgaaat gattggaaaa aataagggta ctgtttcaga cccgctggcc
     4681 gcttaggccg ttgagacttt aaagatctcc gccacgcaaa ccaattcccc gtttcagacc
     4741 cgctggccgc ttaggccgtt gagactgggg gttggggttt ctatttttcg ttagtattca
     4801 gagtttcaga cccgctggcc gcttaggccg ttgagacaag atattatctt cgttatggcg
     4861 gaaattatca atgtttcaga cccgctggcc gcttaggccg ttgagactta ctgaacgaag
     4921 actgggaggc atgggagcag aatgtttcag acccgctggc cgcttaggcc gttgagactt
     4981 tggcggtatt gccatcatct cctcatcaag ctgtttcaga cccgctggcc gcttaggccg
     5041 ttgagacaag taatacttgc tcaagtcttc agctatacga taggtttcag acccgctggc
     5101 cgcttaggcc gttgagactt agatagaagt tgagaacacg tcagatggat taagtcgttt
     5161 cagacccgct ggccgcttag gccgttgaga ctttgacgca taaacctgcg cgcacaagtg
     5221 cgttctgttt cagacccgct ggtcgttgag acgtgtgacg atgaaccatg actgttggat
     5281 tggaacggtt cggtttaact gggggcgatc cgttagacaa gaccccctcc cagccttaag
     5341 taagtgagag gagcgagctt tgttccctcc ccttacttaa ggggagggtt agggaggggt
     5401 ctattaactg ggaacatcgt ttgcggccgc gatcaagcag gtgcgacaga cgtcatacta
     5461 gatatcaagc gacttctcct atcccctggg aacacatcaa tctcaccgga gaatatcgct
     5521 ggccaaagcc ttagcgtagg attccgcccc ttcccgcaaa cgaccccaaa caggaaacgc
     5581 agctgaaacg ggaagctcaa cacccactga cgcatgggtt gttcaggcag tacttcatca
     5641 accagcaagg cggcactttc ggccatccgc cgcgccccac agctcgggca gaaaccgcga
     5701 cgcttacagc tgaaagcgac caggtgctcg gcgtggcaag actcgcagcg aacccgtaga
     5761 aagccatgct ccagccgccc gcattggaga aattcttcaa attcccgttg cacatagccc
     5821 ggcaattcct ttccctgctc tgccataagc gcagcgaatg ccgggtaata ctcgtcaacg
     5881 atctgataga gaagggtttg ctcgggtcgg tggctctggt aacgaccagt atcccgatcc
     5941 cggctggccg tcctggccgc cacatgaggc atgttccgcg tccttgcaat actgtgttta
     6001 catacagtct atcgcttagc ggaaagttct tttaccctca gccgaaatgc ctgccgttgc
     6061 tagacattgc cagccagtgc ccgtcactcc cgtactaact gtcacgaacc cctgcaataa
     6121 ctgtcacgcc cccctgcaat aactgtcacg aacccctgca ataactgtca cgcccccaaa
     6181 cctgcaaacc cagcaggggc gggggctggc ggggtgttgg aaaaatccat ccatgattat
     6241 ctaagaataa tccactaggc gcggttatca gcgcccttgt ggggcgctgc tgcccttgcc
     6301 caatatgccc ggccagaggc cggatagctg gtctattcgc tgcgctaggc tacacaccgc
     6361 cccaccgctg cgcggcaggg ggaaaggcgg gcaaagcccg ctaaacccca caccaaaccc
     6421 cgcagaaata cgctggagcg cttttagccg ctttagcggc ctttccccct acccgaaggg
     6481 tgggggcgcg tgtgcagccc cgcagggcct gtctcggtcg atcattcagc ccggctcatc
     6541 cttctggcgt ggcggcagac cgaacaaggc gcggtcgtgg tcgcgttcaa ggtacgcatc
     6601 cattgccgcc atgagccgat cctccggcca ctcgctgctg ttcaccttgg ccaaaatcat
     6661 ggcccccacc agcaccttgc gccttgtttc gttcttgcgc tcttgctgct gttcccttgc
     6721 ccgcacccgc tgaatttcgg cattgattcg cgctcgttgt tcttcgagct tggccagccg
     6781 atccgccgcc ttgttgctcc ccttaaccat cttgacaccc cattgttaat gtgctgtctc
     6841 gtaggctatc atggaggcac agcggcggca atcccgaccc tactttgtag gggagggcgc
     6901 acttaccggt ttctcttcga gaaactggcc taacggccac ccttcgggcg gtgcgctctc
     6961 cgagggccat tgcatggagc cgaaaagcaa aagcaacagc gaggcagcat ggcgatttat
     7021 caccttacgg cgaaaaccgg cagcaggtcg ggcggccaat cggccagggc caaggccgac
     7081 tacatccagc gcgaaggcaa gtatgcccgc gacatggatg aagtcttgca cgccgaatcc
     7141 gggcacatgc cggagttcgt cgagcggccc gccgactact gggatgctgc cgacctgtat
     7201 gaacgcgcca atgggcggct gttcaaggag gtcgaatttg ccctgccggt cgagctgacc
     7261 ctcgaccagc agaaggcgct ggcgtccgag ttcgcccagc acctgaccgg tgccgagcgc
     7321 ctgccgtata cgctggccat ccatgccggt ggcggcgaga acccgcactg ccacctgatg
     7381 atctccgagc ggatcaatga cggcatcgag cggcccgccg ctcagtggtt caagcggtac
     7441 aacggcaaga ccccggagaa gggcggggca cagaagaccg aagcgctcaa gcccaaggca
     7501 tggcttgagc agacccgcga ggcatgggcc gaccatgcca accgggcatt agagcgggct
     7561 ggccacgacg cccgcattga ccacagaaca cttgaggcgc agggcatcga gcgcctgccc
     7621 ggtgttcacc tggggccgaa cgtggtggag atggaaggcc ggggcatccg caccgaccgg
     7681 gcagacgtgg ccctgaacat cgacaccgcc aacgcccaga tcatcgactt acaggaatac
     7741 cgggaggcaa tagaccatga acgcaatcga cagagtgaag aaatccagag gcatcaacga
     7801 gttagcggag cagatcgaac cgctggccca gagcatggcg acactggccg acgaagcccg
     7861 gcaggtcatg agccagaccc agcaggccag cgaggcgcag gcggcggagt ggctgaaagc
     7921 ccagcgccag acaggggcgg catgggtgga gctggccaaa gagttgcggg aggtagccgc
     7981 cgaggtgagc agcgccgcgc agagcgcccg gagcgcgtcg cgggggtggc actggaagct
     8041 atggctaacc gtgatgctgg cttccatgat gcctacggtg gtgctgctga tcgcatcgtt
     8101 gctcttgctc gacctgacgc cactgacaac cgaggacggc tcgatctggc tgcgcttggt
     8161 ggcccgatga agaacgacag gactttgcag gccataggcc gacagctcaa ggccatgggc
     8221 tgtgagcgct tcgatatcgg cgtcagggac gccaccaccg gccagatgat gaaccgggaa
     8281 tggtcagccg ccgaagtgct ccagaacacg ccatggctca agcggatgaa tgcccagggc
     8341 aatgacgtgt atatcaggcc cgccgagcag gagcggcatg gtctggtgct ggtggacgac
     8401 ctcagcgagt ttgacctgga tgacatgaaa gccgagggcc gggagcctgc cctggtagtg
     8461 gaaaccagcc cgaagaacta tcaggcatgg gtcaaggtgg ccgacgccgc aggcggtgaa
     8521 cttcgggggc agattgcccg gacgctggcc agcgagtacg acgccgaccc ggccagcgcc
     8581 gacagccgcc actatggccg cttggcgggc ttcaccaacc gcaaggacaa gcacaccacc
     8641 cgcgccggtt atcagccgtg ggtgctgctg cgtgaatcca agggcaagac cgccaccgct
     8701 ggcccggcgc tggtgcagca ggctggccag cagatcgagc aggcccagcg gcagcaggag
     8761 aaggcccgca ggctggccag cctcgaactg cccgagcggc agcttagccg ccaccggcgc
     8821 acggcgctgg acgagtaccg cagcgagatg gccgggctgg tcaagcgctt cggtgatgac
     8881 ctcagcaagt gcgactttat cgccgcgcag aagctggcca gccggggccg cagtgccgag
     8941 gaaatcggca aggccatggc cgaggccagc ccagcgctgg cagagcgcaa gcccggccac
     9001 gaagcggatt acatcgagcg caccgtcagc aaggtcatgg gtctgcccag cgtccagctt
     9061 gcgcgggccg agctggcacg ggcaccggca ccccgccagc gaggcatgga caggggcggg
     9121 ccagatttca gcatgtagtg cttgcgttgg tactcacgcc tgttatacta tgagtactca
     9181 cgcacagaag ggggttttat ggaatacgaa aaaagcgctt cagggtcggt ctacctgatc
     9241 aaaagtgaca agggctattg gttgcccggt ggctttggtt atacgtcaaa caaggccgag
     9301 gctggccgct tttcagtcgc tgatatggcc agccttaacc ttgacggctg caccttgtcc
     9361 ttgttccgcg aagacaagcc tttcggcccc ggcaagtttc tcggtgactg atatgaaaga
     9421 ccaaaaggac aagcagaccg gcgacctgct ggccagccct gacgctgtac gccaagcgcg
     9481 atatgccgag cgcatgaagg ccaaagggat gcgtcagcgc aagttctggc tgaccgacga
     9541 cgaatacgag gcgctgcgcg agtgcctgga agaactcaga gcggcgcagg gcgggggtag
     9601 tgaccccgcc agcgcctaac caccaactgc ctgcaaagga ggcaatcaat ggctacccat
     9661 aagcctatca atattctgga ggcgttcgca gcagcgccgc caccgctgga ctacgttttg
     9721 cccaacatgg tggccggtac ggtcggggcg ctggtgtcgc ccggtggtgc cggtaaatcc
     9781 atgctggccc tgcaactggc cgcacagatt gcaggcgggc cggatctgct ggaggtgggc
     9841 gaactgccca ccggcccggt gatctacctg cccgccgaag acccgcccac cgccattcat
     9901 caccgcctgc acgcccttgg ggcgcacctc agcgccgagg aacggcaagc cgtggctgac
     9961 ggcctgctga tccagccgct gatcggcagc ctgcccaaca tcatggcccc ggagtggttc
    10021 gacggcctca agcgcgccgc cgagggccgc cgcctgatgg tgctggacac gctgcgccgg
    10081 ttccacatcg aggaagaaaa cgccagcggc cccatggccc aggtcatcgg tcgcatggag
    10141 gccatcgccg ccgataccgg gtgctctatc gtgttcctgc accatgccag caagggcgcg
    10201 gccatgatgg gcgcaggcga ccagcagcag gccagccggg gcagctcggt actggtcgat
    10261 aacatccgct ggcagtccta cctgtcgagc atgaccagcg ccgaggccga ggaatggggt
    10321 gtggacgacg accagcgccg gttcttcgtc cgcttcggtg tgagcaaggc caactatggc
    10381 gcaccgttcg ctgatcggtg gttcaggcgg catgacggcg gggtgctcaa gcccgccgtg
    10441 ctggagaggc agcgcaagag caagggggtg ccccgtggtg aagcctaaga acaagcacag
    10501 cctcagccac gtccggcacg acccggcgca ctgtctggcc cccggcctgt tccgtgccct
    10561 caagcggggc gagcgcaagc gcagcaagct ggacgtgacg tatgactacg gcgacggcaa
    10621 gcggatcgag ttcagcggcc cggagccgct gggcgctgat gatctgcgca tcctgcaagg
    10681 gctggtggcc atggctgggc ctaatggcct agtgcttggc ccggaaccca agaccgaagg
    10741 cggacggcag ctccggctgt tcctggaacc caagtgggag gccgtcaccg ctgaatgcca
    10801 tgtggtcaaa ggtagctatc gggcgctggc aaaggaaatc ggggcagagg tcgatagtgg
    10861 tggggcgctc aagcacatac aggactgcat cgagcgcctt tggaaggtat ccatcatcgc
    10921 ccagaatggc cgcaagcggc aggggtttcg gctgctgtcg gagtacgcca gcgacgaggc
    10981 ggacgggcgc ctgtacgtgg ccctgaaccc cttgatcgcg caggccgtca tgggtggcgg
    11041 ccagcatgtg cgcatcagca tggacgaggt gcgggcgctg gacagcgaaa ccgcccgcct
    11101 gctgcaccag cggctgtgtg gctggatcga ccccggcaaa accggcaagg cttccataga
    11161 taccttgtgc ggctatgtct ggccgtcaga ggccagtggt tcgaccatgc gcaagcgccg
    11221 ccagcgggtg cgcgaggcgt tgccggagct ggtcgcgctg ggctggacgg taaccgagtt
    11281 cgcggcgggc aagtacgaca tcacccggcc caaggcggca ggctgacccc ccccactcta
    11341 ttgtaaacaa gacattttta tcttttatat tcaatggctt attttcctgc taattggtaa
    11401 taccatgaaa aataccatgc tcagaaaagg cttaacaata ttttgaaaaa ttgcctactg
    11461 agcgctgccg cacagctcca taggccgctt tcctggcttt gcttccagat gtatgctctt
    11521 ctgctcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    11581 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    11641 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    11701 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    11761 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    11821 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    11881 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    11941 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccttaataa
    12001 gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa
    12061 ccgccttgca gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta
    12121 actggcttgg aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc
    12181 atgacttcaa gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt
    12241 gcatgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac
    12301 tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt
    12361 gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa
    12421 ggcaggaaca ggagagcgca cgagggagcc gccaggggga aacgcctggt atctttatag
    12481 tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg
    12541 gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt aagtatcttc
    12601 ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga
    12661 acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta tcacatattc
    12721 tgctgacgca ccggtgcagc cttttttctc ctgccacatg aagcacttca ctgacaccct
    12781 catcagtgcc aacatagtaa gccagtatac actccgctag cgctgaggtc tgcctcgtga
    12841 agaaggtgtt gctgactcat accaggcctg aatcgcccca tcatccagcc agaaagtgag
    12901 ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt tgaacttttg
    12961 ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct tcaactcagc
    13021 aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat gttacattgc
    13081 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
    13141 aaggggtgtt atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc
    13201 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
    13261 tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
    13321 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
    13381 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
    13441 caccactgcg atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg
    13501 tgaaaatatt gctgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
    13561 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
    13621 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
    13681 agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg tcactcatgg
    13741 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
    13801 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
    13861 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
    13921 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
    13981 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcggct ttgttgaata
    14041 aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt
    14101 tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcatc
    14161 aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcctg gtatgagtca
    14221 gcaacacctt cttcacgagg cagacctcag cgctcaaaga tgcaggggta aaagctaacc
    14281 gcatctttac cgacaaggca tccggcagtt caacagatcg ggaagggctg gatttgctga
    14341 ggatgaaggt ggaggaaggt gatgtcattc tggtgaagaa gctcgaccgt cttggccgcg
    14401 acacgccgac atgatccaac tgataaaaga gtttgatgct cagggtgtag cggttcggtt
    14461 tattgacgac gggatcagta ccgacggtga tatggggcaa atggtggtca ccatcctgtc
    14521 ggctgtggca caggctgaac gccggaggat cctagagcgc acgaatgagg gccgacagga
    14581 agcaaagctg aaaggaatca aatttggccg caggcgtacc gtggacagga acgtcgtgct
    14641 gacgcttcat cagaagggca ctggtgcaac ggaaattgct catcagctca gtattgcccg
    14701 ctccacggtt tataaaattc ttgaagacga aagggcctcg tgcatacgcc tatttttata
    14761 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
    14821 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
    14881 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
    14941 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
    15001 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacggc aacaaaatca
    15061 aatgtgaagt tgccctagta accggtaaaa aagatcacga taaacgtgat actgccaaag
    15121 aacgcgactg ggcgcgcgat aaagaacgcc taatgaaaca cagcgtataa acgctatgta
    15181 cggttttgct agcgttaacg atctgttttc gttagaattg ccgatacttt gggggtgaca
    15241 tggtttcgac gctggtcacg aaccctgagg tgcatgtcga gagtgatacg tgatctcttg
    15301 ggttaattga ggcctgagta taaggtgact tatacttgta atctatctaa acggggaacc
    15361 tctctagtag acaatcccgt gctaaattgt aggactgccc tttaataaat acttctatat
    15421 ttaaagaggt atttatgaaa agcggaattt atcagattaa aaatactttc tctagagaaa
    15481 atttcgtctg gattagttac ttatcgtgta aaatctgata aatggaattg gttctacata
    15541 aatgcctaac gactatccct ttggggagta gggtcaagtg actcgaaacg atagacaact
    15601 tgctttaaca agttggagat atagtctgct ctgcatggtg acatgcagct ggatataatt
    15661 ccggggtaag attaacgacc ttatctgaac ataatgctcc tcgtatcaat tatatagtcg
    15721 caaacgacga aaactacgct ttagcagctt aataacctgc tagtgtgctg ccctcaggtt
    15781 gcttgtagcc cgagattccg cagtatcata aattacaaga tgctactcga tgccctgcct
    15841 ggggataaga gtataagatc aagcaggctc gccagtaaca ccctgtcctt cgggcggttc
    15901 ctggtcaaaa aatagaagtg actaaacatg tagagccgat ggcagaggac tggcggacgg
    15961 gggttcgacc ccccccacct ccaccaaata aaggtttgat tttaaaagag ttttagtgca
    16021 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
    16081 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
    16141 cgtgtt
//
