LOCUS       sequence-146317-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express C-terminal YN fused flock house virus (FHV) B2 protein 
            (2741-2956 nt) for dsRNA labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Cheng X, Deng P, Cui H, Wang A
  TITLE     Visualizing double-stranded RNA distribution and dynamics in living 
            cells by dsRNA binding-dependent fluorescence complementation.
  JOURNAL   Virology. 2015 Nov;485:439-51. doi: 10.1016/j.virol.2015.08.023. 
            Epub 2015 Sep 7.
  PUBMED    26351203
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2015-11"; volume: "485:439-51. doi"; pages: " 
            10.1016/j.virol.2015.08.023. Epub 2015 Sep 7"
COMMENT     Sequence Label: pdRBFC-B2-YN
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    30..84
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             89..115
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    119..143
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(369..393)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(458..479)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(719..738)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgctcgagta taagagctct atttttacaa caattaccaa caacaacaaa caacaaacaa
       61 cattacaatt acatttacaa ttaccatgta cccatacgat gttccagatt acgctatcac
      121 aagtttgtac aaaaaagcag gctgcatgat gccaagcaaa ctcgcgctaa tccaggaact
      181 tcccgaccgc attcaaacgg cggtggaagc agccatggga atgagctacc aagacgcacc
      241 gaacaacgtg cgcagggacc tcgacaacct gcacgcttgc ctaaacaagg caaaactaac
      301 ggtaagtcgg atggtaacat cactgctgga gaaacccagc gtggtggcat acctagaggg
      361 aaaggccgac ccagctttct tgtacaaagt ggtgcctagg agtatcgcta caatggtgag
      421 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      481 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      541 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      601 caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
      661 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      721 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      781 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
      841 gtacaactac aacagccaca acgtctatat catgg
//
