LOCUS       sequence-146336-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express C-terminal YC fused flock house virus (FHV) B2 protein 
            (2741-2956 nt) for dsRNA labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Cheng X, Deng P, Cui H, Wang A
  TITLE     Visualizing double-stranded RNA distribution and dynamics in living 
            cells by dsRNA binding-dependent fluorescence complementation.
  JOURNAL   Virology. 2015 Nov;485:439-51. doi: 10.1016/j.virol.2015.08.023. 
            Epub 2015 Sep 7.
  PUBMED    26351203
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2015-11"; volume: "485:439-51. doi"; pages: " 
            10.1016/j.virol.2015.08.023. Epub 2015 Sep 7"
COMMENT     Sequence Label: pdRBFC-B2-YC
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    30..84
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             89..112
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    116..140
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(366..390)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     577..598
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgctcgagta taagagctct atttttacaa caattaccaa caacaacaaa caacaaacaa
       61 cattacaatt acatttacaa ttaccatgga ctacaaagac gatgacgaca aaatcacaag
      121 tttgtacaaa aaagcaggct gcatgatgcc aagcaaactc gcgctaatcc aggaacttcc
      181 cgaccgcatt caaacggcgg tggaagcagc catgggaatg agctaccaag acgcaccgaa
      241 caacgtgcgc agggacctcg acaacctgca cgcttgccta aacaaggcaa aactaacggt
      301 aagtcggatg gtaacatcac tgctggagaa acccagcgtg gtggcatacc tagagggaaa
      361 ggccgaccca gctttcttgt acaaagtggt gcctaggcct gcttgtaaga tccctaacga
      421 cctcaagcag aaagtcatga accacggcag cgtgcagctc gccgaccact accagcagaa
      481 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
      541 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
      601 cgccgccggg atcactctcg gcatggacga gctgtacaag tgacctaggt gagtctagag
      661 agttaattct aggtgagtct agagagttaa ttaagacccg ggactagtcc ctagagtcct
      721 gctttaatga gatatgcgag acgcctatga tcgcatgata tttgctttca attctgttgt
      781 gcacgttgta aa
//
