LOCUS       sequence-146339-         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression plasmid for SpCas9-HF1 variant: 
            CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Kleinstiver BP, Pattanayak V, Prew MS, Tsai SQ, Nguyen NT, Zheng Z, 
            Keith Joung J
  TITLE     High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide 
            off-target effects.
  JOURNAL   Nature. 2016 Jan 6. doi: 10.1038/nature16526.
  PUBMED    26735016
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Jan 6. doi: 10.1038/nature16526."
COMMENT     Sequence Label: VP12
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aacgactcag aaggggcaaa aaaacagtcg agagcggatg aagagaatag aagagggtat
       61 taaagaactg ggcagccaga tcttaaagga gcatcctgtg gaaaataccc aattgcagaa
      121 cgagaaactt tacctctatt acctacaaaa tggaagggac atgtatgttg atcaggaact
      181 ggacataaac cgtttatctg attacgacgt cgatcacatt gtaccccaat cctttttgaa
      241 ggacgattca atcgacaata aagtgcttac acgctcggat aagaaccgag ggaaaagtga
      301 caatgttcca agcgaggaag tcgtaaagaa aatgaagaac tattggcggc agctcctaaa
      361 tgcgaaactg ataacgcaaa gaaagttcga taacttaact aaagctgaga ggggtggctt
      421 gtctgaactt gacaaggccg gatttattaa acgtcagctc gtggaaaccc gcgccatcac
      481 aaagcatgtt gcgcagatac tagattcccg aatgaatacg aaatacgacg agaacgataa
      541 gctgattcgg gaagtcaaag taatcacttt aaagtcaaaa ttggtgtcgg acttcagaaa
      601 ggattttcaa ttctataaag ttagggagat aaataactac caccatgcgc acgacgctta
      661 tcttaatgcc gtcgtaggga ccgcactcat taagaaatac ccgaagctag aaagtgagtt
      721 tg
//
