LOCUS       sequence-146342-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  I-SceI endonuclease expression vector with mammalian promoter to 
            introduce a DSB at a genomic I-SceI site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Richardson C, Moynahan ME, Jasin M
  TITLE     Double-strand break repair by interchromosomal recombination: 
            suppression of chromosomal translocations.
  JOURNAL   Genes Dev. 1998 Dec 15. 12(24):3831-42.
  PUBMED    9869637
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            1998 Dec 15. 12(24):3831-42."
COMMENT     Sequence Label: pCBASceI
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             123..143
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             165..185
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             204..230
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc
       61 tcatcatttt ggcaaagaat tcctcgaaat tcagatccgc cgccactatg ggatcaagat
      121 cgccaaaaaa gaagagaaag gtgccgaaga agcatgcagc accaccaaaa aaaaaacgaa
      181 aagtagaaga cccacgattt atgtacccat acgatgttcc tgactatgcg ggtatgaaaa
      241 acatcaaaaa aaaccaggta atgaacctgg gtccgaactc taaactgctg aaagaataca
      301 aatcccagct gatcgaactg aacatcgaac agttcgaagc aggtatcggt ctgatcctgg
      361 gtgatgctta catccgttct cgtgatgaag gtaaaaccta ctgtatgcag ttcgagtgga
      421 aaaacaaagc atacatggac cacgtatgtc tgctgtacga tcagtgggta ctgtccccgc
      481 cgcacaaaaa agaacgtgtt aaccacctgg gtaacctggt aatcacctgg ggcgcccaga
      541 ctttcaaaca ccaagctttc aacaaactgg ctaacctgtt catcgttaac aacaaaaaaa
      601 ccatcccgaa caacctggtt gaaaactacc tgaccccgat gtctctggca tactggttca
      661 tggatgatgg tggtaaatgg gattacaaca aaaactctac caacaaatcg atcgtactga
      721 acacccagtc tttcactttc gaagaagtag aatacctggt taagg
//
