LOCUS       sequence-146344-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express C-terminal YC fused influenza A virus partial NS1 protein 
            (27-236 nt) for dsRNA labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Cheng X, Deng P, Cui H, Wang A
  TITLE     Visualizing double-stranded RNA distribution and dynamics in living 
            cells by dsRNA binding-dependent fluorescence complementation.
  JOURNAL   Virology. 2015 Nov;485:439-51. doi: 10.1016/j.virol.2015.08.023. 
            Epub 2015 Sep 7.
  PUBMED    26351203
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2015-11"; volume: "485:439-51. doi"; pages: " 
            10.1016/j.virol.2015.08.023. Epub 2015 Sep 7"
COMMENT     Sequence Label: pdRBFC-NS1-YC
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    30..84
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             89..112
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    116..140
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(357..381)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     568..589
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgctcgagta taagagctct atttttacaa caattaccaa caacaacaaa caacaaacaa
       61 cattacaatt acatttacaa ttaccatgga ctacaaagac gatgacgaca aaatcacaag
      121 tttgtacaaa aaagcaggct tcatgatgga tccaaacact gtgtcaagct ttcaggtaga
      181 ttgctttctt tggcatgtcc gcaaacgagt tgcagaccaa gaactaggtg atgccccatt
      241 ccttgatcgg cttcgccgag atcagaaatc cctaagagga aggggcagca ccctcggtct
      301 ggacatcgag acagccacac gtgctggaaa gcagatagtg gagcggattc tgaaagaccc
      361 agctttcttg tacaaagtgg tgcctaggcc tgcttgtaag atccctaacg acctcaagca
      421 gaaagtcatg aaccacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      481 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag
      541 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      601 gatcactctc ggcatggacg agctgtacaa gtgacctagg tgagtctaga gagttaattc
      661 taggtgagtc tagagagtta attaagaccc gggactagtc cctagagtcc tgctttaatg
      721 agatatgcga gacgcctatg atcgcatgat atttgctttc aattctgttg tgcacgttgt
      781 aaaa
//
