LOCUS       sequence-146352-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pUC plasmid with inverted polylinker flanking PpsbA-Km marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Black TA, Wolk CP
  TITLE     Analysis of a Het- mutation in Anabaena sp. strain PCC 7120 
            implicates a secondary metabolite in the regulation of heterocyst 
            spacing.
  JOURNAL   J Bacteriol. 1994 Apr;176(8):2282-92.
  PUBMED    8157596
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol."; date: "1994-04"; volume: "176(8)"; pages: "2282-92"
COMMENT     Sequence Label: pRL648
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(180..199)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     790..809
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ctcggtaccc ggggatcctc tagaggatct caatgaatat tggttgacac gggcgtataa
       61 gacatgttat actgttgaat aacaaggacg gatctgatca agagacagga tgaggatcgt
      121 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
      181 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
      241 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
      301 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
      361 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
      421 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
      481 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
      541 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
      601 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
      661 ccgacggcga tgatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
      721 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
      781 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggc
//
