LOCUS       sequence-146355-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express C-terminal YN fused Marburg virus partial VP35 protein 
            (3541-3930 nt) for dsRNA labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Cheng X, Deng P, Cui H, Wang A
  TITLE     Visualizing double-stranded RNA distribution and dynamics in living 
            cells by dsRNA binding-dependent fluorescence complementation.
  JOURNAL   Virology. 2015 Nov;485:439-51. doi: 10.1016/j.virol.2015.08.023. 
            Epub 2015 Sep 7.
  PUBMED    26351203
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2015-11"; volume: "485:439-51. doi"; pages: " 
            10.1016/j.virol.2015.08.023. Epub 2015 Sep 7"
COMMENT     Sequence Label: pdRBFC-VP35-YN
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    30..84
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             89..115
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    119..143
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(528..552)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(617..638)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cgctcgagta taagagctct atttttacaa caattaccaa caacaacaaa caacaaacaa
       61 cattacaatt acatttacaa ttaccatgta cccatacgat gttccagatt acgctatcac
      121 aagtttgtac aaaaaagcag gcttcatgtc caagccaaat ctttcagcta aggatttagc
      181 ccttttattg tttacccatc tacccggcaa caacactcca ttccatatcc tagctcaggt
      241 cctttcaaaa attgcttaca agtcaggaaa atccggagca ttcttggatg catttcacca
      301 gattctaagt gaaggagaga atgctcaggc ggcattaact cgactaagca gaacatttga
      361 cgctttcctt ggagtggttc ctccagtgat aagagtcaaa aacttccaaa cagtccctcg
      421 tccatgtcaa aaaagtcttc gggctgtccc tccaaatcca acaattgaca aaggatgggt
      481 ctgtgtttat tcatctgagc aaggtgagac acgggccctg aaaatcgacc cagctttctt
      541 gtacaaagtg gtgcctagga gtatcgctac aatggtgagc aagggcgagg agctgttcac
      601 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      661 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      721 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca
      781 gtgcttcgcc cgctaccc
//
