LOCUS       sequence-146360-        3410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Hopx. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Hopx
FEATURES             Location/Qualifiers
     source          1..3410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(535..553)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(536..553)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(571..587)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(571..587)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(584..606)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    595..611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(619..625,626..643,644..649))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    664..685
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(802..819)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(973..1561)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1053..1072)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1732..2523,2524..2592))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2355..2374
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2593..2697)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2775..3230)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2912..2933)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3124..3143
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3356..3378
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3370..3387
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3371..3387
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3394..3410,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3394..3410,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tatctccgga ggcagcactt gaggcgcttc ctcagtatac tgtcccctcg gagtgtcagg
      121 cgggaggcgt cttcttcctc ctctccatcc ttagtcagac gcgcacggac catgtcggcg
      181 cagaccgcga gcggccccac ggaggaccag gtggagatcc tggagtacaa cttcaacaag
      241 gtcaacaagc acccggaccc caccacgctg tgcctcatcg cagccgaggc gggtctcacg
      301 gaggagcaga cgcagaaatg gtttaagcag cgcctggcag agtggcggcg gtcagaaggc
      361 ttgccttcgg aatgcagatc tgttacggac tagggagcca ggcccttgag cttgctcttg
      421 gaactccatc tcttcttcct tccctcggct taccaatcac tagtgaattc gcggccgcct
      481 gcaggtcgac catatgggag agctcccaac gcgttggatg catagcttga gtattctata
      541 gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
      601 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
      661 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
      721 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
      781 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      841 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      901 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      961 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1021 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1081 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1141 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1201 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1261 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     1321 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     1381 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1441 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1501 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1561 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1621 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1681 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1741 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1801 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1861 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     1921 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1981 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     2041 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     2101 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     2161 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     2221 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     2281 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     2341 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     2401 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     2461 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     2521 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     2581 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     2641 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     2701 atttccccga aaagtgccac ctgatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     2761 aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     2821 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     2881 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     2941 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     3001 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     3061 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     3121 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     3181 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca
     3241 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     3301 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     3361 tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
//
