LOCUS       sequence-146366-         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express C-terminal YC fused Marburg virus partial VP35 protein 
            (3541-3930 nt) for dsRNA labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Cheng X, Deng P, Cui H, Wang A
  TITLE     Visualizing double-stranded RNA distribution and dynamics in living 
            cells by dsRNA binding-dependent fluorescence complementation.
  JOURNAL   Virology. 2015 Nov;485:439-51. doi: 10.1016/j.virol.2015.08.023. 
            Epub 2015 Sep 7.
  PUBMED    26351203
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2015-11"; volume: "485:439-51. doi"; pages: " 
            10.1016/j.virol.2015.08.023. Epub 2015 Sep 7"
COMMENT     Sequence Label: pdRBFC-VP35-YC
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    30..84
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             89..112
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    116..140
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(525..549)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     736..757
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgctcgagta taagagctct atttttacaa caattaccaa caacaacaaa caacaaacaa
       61 cattacaatt acatttacaa ttaccatgga ctacaaagac gatgacgaca aaatcacaag
      121 tttgtacaaa aaagcaggct tcatgtccaa gccaaatctt tcagctaagg atttagccct
      181 tttattgttt acccatctac ccggcaacaa cactccattc catatcctag ctcaggtcct
      241 ttcaaaaatt gcttacaagt caggaaaatc cggagcattc ttggatgcat ttcaccagat
      301 tctaagtgaa ggagagaatg ctcaggcggc attaactcga ctaagcagaa catttgacgc
      361 tttccttgga gtggttcctc cagtgataag agtcaaaaac ttccaaacag tccctcgtcc
      421 atgtcaaaaa agtcttcggg ctgtccctcc aaatccaaca attgacaaag gatgggtctg
      481 tgtttattca tctgagcaag gtgagacacg ggccctgaaa atcgacccag ctttcttgta
      541 caaagtggtg cctaggcctg cttgtaagat ccctaacgac ctcaagcaga aagtcatgaa
      601 ccacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
      661 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa
      721 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
      781 catggacgag ctgtacaagt gacct
//
