LOCUS       sequence-146368-        3908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Pbx2. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Pbx2
FEATURES             Location/Qualifiers
     source          1..3908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1033..1051)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1034..1051)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1069..1085)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1069..1085)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1082..1104)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1093..1109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1117..1123,1124..1141,1142..1147))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1162..1183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1300..1317)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1471..2059)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1551..1570)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2230..3021,3022..3090))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2853..2872
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3091..3195)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3273..3728)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3410..3431)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3622..3641
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3854..3876
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3868..3885
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3869..3885
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3892..3908,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3892..3908,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 taaaagtcca actccaaagc ctagtcaaca gttttctaaa gtagcagaaa ctccctccga
      121 ggtagatatc cgagtcagac actcttgtcc accgagcggt tctattggtt taagatgagc
      181 tgcgtatgag gtaataaagc cgtctggagg ggcaggaggt gggggtgcat gggggagggg
      241 gtgttggccc agggcctctg tccaagagtc cccatttttg gcatctctgg gtcgggcagg
      301 gctgacatct ccagattcca gagtatataa gtggggtggg gaacagaagt aggaggagtc
      361 caagacaaac acaactggtg ttaggttgta gatttaagag agacatctga gaaataggta
      421 tctggttttg gtgagaggtg agacgaaaaa aagaaagaag aaatggggac accccactct
      481 ccctgccgtg ttgtcagcac cccagcacca agcgtctggt cggggagggc acaaccccaa
      541 tcgccctctg ttttgtgctt ctgtggggtt tgtcctctag atgcccagag tccttaagag
      601 ttgcctggtg gaagccccta gccccgcgac cttgaggggc aaggccagtt ggaggtgtca
      661 gagtgaacac ttcctggtcc ctctgtcggg gatgtcaccg aggacggggt cacagcctcc
      721 tgccagccac cattggcccg aatttctcga ggactgtata tctgacctcc cccaatgtta
      781 tccccgtagc tccccggccc catcgagtgt cggagtgatt ccacctggga agcagggtag
      841 gaatcgccat tgagcccggg catccccaga aacatgtctc cggatcctga gagattgaaa
      901 gagccgccag agcctgcaga ggaagggggc gttggagagc tggtgcggct gtaatcacta
      961 gtgaattcgc ggccgcctgc aggtcgacca tatgggagag ctcccaacgc gttggatgca
     1021 tagcttgagt attctatagt gtcacctaaa tagcttggcg taatcatggt catagctgtt
     1081 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     1141 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     1201 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     1261 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     1321 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1381 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1441 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1501 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1561 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1621 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1681 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1741 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1801 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1861 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     1921 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1981 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2041 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2101 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2161 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     2221 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2281 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2341 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     2401 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2461 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2521 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     2581 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     2641 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     2701 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     2761 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     2821 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     2881 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     2941 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3001 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3061 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     3121 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3181 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gatgcggtgt gaaataccgc
     3241 acagatgcgt aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa
     3301 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     3361 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     3421 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     3481 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     3541 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     3601 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     3661 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     3721 gggcgcgtcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     3781 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     3841 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac
     3901 tcactata
//
