LOCUS       sequence-146369-        3731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Gap43. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Hirota J, Mombaerts P
  TITLE     The LIM-homeodomain protein Lhx2 is required for complete 
            development of mouse olfactory sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jun 8;101(23):8751-5. Epub 2004 Jun 
            1.
  PUBMED    15173589
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2004-06-8"; volume: "101(23)"; pages: 
            "8751-5. Epub 2004 Jun 1"
COMMENT     Sequence Label: pISH-Gap43
FEATURES             Location/Qualifiers
     source          1..3731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(856..874)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(857..874)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(892..908)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(892..908)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(905..927)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    916..932
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(940..946,947..964,965..970))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    985..1006
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1123..1140)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1294..1882)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1374..1393)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2053..2844,2845..2913))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2676..2695
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2914..3018)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3096..3551)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3233..3254)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3445..3464
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3677..3699
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3691..3708
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3692..3708
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3715..3731,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3715..3731,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 taccatgctg tgctgtatga gaagaaccaa acaggttgaa aagaatgatg aggaccaaat
      121 gattgaacaa gatggtgtca agccggaaga taaggctcat aaggctgcga ccaaaattca
      181 ggctagcttc cgtggacaca taacaaggaa aaagctcaaa ggcgagaaga agggtgatgc
      241 accagctgct gaggccgagg ccaaggagaa ggatgatgct cccgttgctg atggtgtgga
      301 gaagaaggag ggagatggct ctgctactac cgatgcagcc ccagccacca gccccaaggc
      361 tgaggagccc agcaaggcag gagatgcacc ttctgaggag aagaagggtg aaggggatgc
      421 ggccccctcc gaggaaaagg ccggctcagc ggagacagaa agtgctgcta aagctaccac
      481 tgataactcc ccgtcctcca aggctgaaga tggcccagcc aaggaggagc ctaaacaagc
      541 cgatgtgcct gctgctgtca ctgatgctgc tgccaccacc cctgctgcag aggatgctgc
      601 caccaaggca gcgcagcctc caacggagac tgcagaaagc agccaagctg aggaggagaa
      661 agacgctgta gacgaagcca aacctaagga aagtgcccga caggatgagg gtaaagaaga
      721 ccccgaggct gaccaagaac atgcctgaac tttaagaaat ggctttccac gttgcaatca
      781 ctagtgaatt cgcggccgcc tgcaggtcga ccatatggga gagctcccaa cgcgttggat
      841 gcatagcttg agtattctat agtgtcacct aaatagcttg gcgtaatcat ggtcatagct
      901 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      961 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     1021 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     1081 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     1141 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     1201 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1261 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1321 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1381 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1441 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1501 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1561 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1621 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1681 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1741 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1801 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1861 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1921 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1981 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2041 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2101 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2161 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     2221 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2281 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2341 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2401 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2461 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2521 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2581 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2641 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     2701 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     2761 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     2821 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     2881 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2941 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     3001 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgatgcgg tgtgaaatac
     3061 cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta atattttgtt
     3121 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     3181 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     3241 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     3301 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     3361 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     3421 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     3481 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     3541 acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     3601 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     3661 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac
     3721 gactcactat a
//
