LOCUS       sequence-146400-        3914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Meis1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Meis1
FEATURES             Location/Qualifiers
     source          1..3914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1039..1057)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1040..1057)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1075..1091)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1075..1091)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1088..1110)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1099..1115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1123..1129,1130..1147,1148..1153))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1168..1189
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1306..1323)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1477..2065)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1557..1576)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2236..3027,3028..3096))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2859..2878
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3097..3201)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3279..3734)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3416..3437)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3628..3647
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3860..3882
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3874..3891
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3875..3891
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3898..3914,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3898..3914,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tgaatccacc tctccgcctg tgcaacacac actttacaca cgcacggcga ctgcaagcgg
      121 gcagcatcga tcgtggctcc tttaagacaa actcagacag acattttttt aaccctcctc
      181 tctaatctcc cttcagtgca gcagttgcaa agagggagag agaaagagaa agagagcgag
      241 agaagagaga aactgattag gaattaggac tgattcaagg aaagcgggcg ctagggcttt
      301 gtgcatttga atattaacat ttgaggtgtt ctgaccagaa gaagacagaa cggacgatca
      361 ttcattcacc acgttgacaa cctcgcctgt gattgacagc tggagtggca gaaagccatg
      421 agatttggta gttgggtctg aggggcgctc tttttttttt ttctttttct tttcttttct
      481 ttcttttttt ttaaactgat ttttttgggg gggagagaag atctgctttt tttttcccct
      541 cccactgctg tcttggtgga accagagcgc ttttatgctc agcgacgcga gcgccttgct
      601 tcaggtccgg tagaccgaag atctgggacc agtaattcac actgctggag acgcaaaggg
      661 attttttttt gttattgttg tgctttattt ttttttccgg gggagtttgc atatttgttt
      721 cttttcacac tggccttaaa gaggatatat tagaagttga agtaggaagg gagccagaga
      781 ggccgatggc gcaaaggtac gacgacctac cccattatgg gggtatggat ggagtaggca
      841 tcccctccac gatgtatggg gacccgcatg cagccaggtc catgcaaccg gtccaccacc
      901 tgaaccacgg gcctcctctg cactcgcatc agtacccgca cacagctcac accaacgcaa
      961 tcactagtga attcgcggcc gcctgcaggt cgaccatatg ggagagctcc caacgcgttg
     1021 gatgcatagc ttgagtattc tatagtgtca cctaaatagc ttggcgtaat catggtcata
     1081 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     1141 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     1201 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     1261 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     1321 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1381 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1441 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1501 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1561 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1621 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1681 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1741 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1801 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1861 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1921 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1981 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2041 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2101 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     2161 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     2221 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     2281 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     2341 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     2401 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     2461 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     2521 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     2581 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     2641 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     2701 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     2761 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     2821 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     2881 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     2941 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     3001 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     3061 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     3121 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     3181 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgatg cggtgtgaaa
     3241 taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg ttaatatttt
     3301 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     3361 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     3421 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     3481 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     3541 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     3601 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     3661 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     3721 gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     3781 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     3841 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa
     3901 tacgactcac tata
//
