LOCUS       sequence-146401-        3903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Pax6. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Pax6
FEATURES             Location/Qualifiers
     source          1..3903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1028..1046)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1029..1046)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1064..1080)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1064..1080)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1077..1099)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1088..1104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1112..1118,1119..1136,1137..1142))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1157..1178
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1295..1312)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1466..2054)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1546..1565)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2225..3016,3017..3085))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2848..2867
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3086..3190)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3268..3723)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3405..3426)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3617..3636
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3849..3871
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3863..3880
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3864..3880
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3887..3903,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3887..3903,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tgggagtgcc cttccatctt tgcttgggaa atccgagaca gattattatc cgagggggtc
      121 tgtaccaacg ataacatacc cagtgtgtca tcaataaaca gagttcttcg caacctggct
      181 agcgaaaagc aacagatggg cgcagacggc atgtatgata aactaaggat gttgaacggg
      241 cagaccggaa gctggggcac acgccctggt tggtatcccg ggacttcagt accagggcaa
      301 cccacgcaag atggctgcca gcaacaggaa ggagggggag agaacaccaa ctccatcagt
      361 tctaacggag aagactcgga tgaagctcag atgcgacttc agctgaagcg gaagctgcaa
      421 agaaatagaa catcttttac ccaagagcag attgaggctc tggagaaaga gtttgagagg
      481 acccattatc cagatgtgtt tgcccgggaa agactagcag ccaaaataga tctacctgaa
      541 gcaagaatac aggtatggtt ttctaatcga agggccaaat ggagaagaga agagaaactg
      601 aggaaccaga gaagacaggc cagcaacact cctagtcaca ttcctatcag cagcagcttc
      661 agtaccagtg tctaccagcc aatcccacag cccaccacac ctgtctcctc cttcacatca
      721 ggttccatgt tgggccgaac agacaccgcc ctcaccaaca cgtacagtgc tttgccaccc
      781 atgcccagct tcaccatggc aaacaacctg cctatgcaac ccccagtccc cagtcagacc
      841 tcctcatact cgtgcatgct gcccaccagc ccgtcagtga atgggcggag ttatgatacc
      901 tacacccctc cgcacatgca aacacacatg aacagtcagc ccatgggaat cactagtgaa
      961 ttcgcggccg cctgcaggtc gaccatatgg gagagctccc aacgcgttgg atgcatagct
     1021 tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg
     1081 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     1141 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     1201 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     1261 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     1321 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1381 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1441 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1501 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1561 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1621 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1681 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1741 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1801 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1861 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1921 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1981 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2041 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2101 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     2161 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     2221 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2281 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2341 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     2401 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2461 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2521 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2581 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2641 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2701 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2761 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2821 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2881 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2941 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3001 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3061 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3121 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     3181 gggttccgcg cacatttccc cgaaaagtgc cacctgatgc ggtgtgaaat accgcacaga
     3241 tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg
     3301 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     3361 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     3421 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     3481 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     3541 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     3601 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     3661 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     3721 cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     3781 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     3841 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact
     3901 ata
//
