LOCUS       sequence-146404-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9-2xNLS-EGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Nelles DA, Fang MY, O'Connell MR, Xu JL, Markmiller SJ, Doudna JA, 
            Yeo GW
  TITLE     Programmable RNA Tracking in Live Cells with CRISPR/Cas9.
  JOURNAL   Cell. 2016 Mar 16. pii: S0092-8674(16)30204-5. doi: 
            10.1016/j.cell.2016.02.054.
  PUBMED    26997482
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2016 
            Mar 16. pii: S0092-8674(16)30204-5. doi: 
            10.1016/j.cell.2016.02.054."
COMMENT     Sequence Label: pCDNA3.1- dCas9-2xNLS-EGFP
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..58)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(38..64)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccttgcgttt tttcttgggg gagccgctgc ccaggctggc ataatcgggc acgtcatagg
       61 gataggcgtc gcctcccagc tgagacaggt cgatccgtgt ctcgtacagg ccggtgatgc
      121 tctggtggat cagggtggcg tccagcacct ctttggtgct ggtgtacctc ttccggtcga
      181 tggtggtgtc aaagtacttg aaggcggcag gggctcccag attggtcagg gtaaacaggt
      241 ggatgatatt ctcggcctgc tctctgatag gcttgtctct gtgcttgttg taggcgctca
      301 gcaccttgtc cagattagcg tcggccagga tcactctctt ggagaactcg ctgatctgct
      361 cgatgatctc gtccaggtag tgtttgtgct gttccacaaa cagctgtttc tgctcattat
      421 cctcggggga gcccttcagc ttctcatagt ggctggccag gtacaggaag ttcacatatt
      481 tggagggcag ggccagttcg tttcccttct gcagttcgcc ggcagaggcc agcattctct
      541 tccggccgtt ttccagctcg aacagggagt acttaggcag cttgatgatc aggtcctttt
      601 tcacttcttt gtagcccttg gcttccagaa agtcgatggg attcttctcg aagctgcttc
      661 tttccatgat ggtgatcccc agcagctctt tcacactctt cagtttcttg gacttgccct
      721 tttccacttt ggccaccacc agcacagaat aggccacggt ggggctgtcg aagccgccgt
      781 acttctta
//
