LOCUS       sequence-146409-         532 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of GFP under the sulA promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Cui L, Bikard D
  TITLE     Consequences of Cas9 cleavage in the chromosome of Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 8. pii: gkw223.
  PUBMED    27060147
REFERENCE   2  (bases 1 to 532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 8. pii: gkw223."
COMMENT     Sequence Label: pZA31-sulA-GFP
FEATURES             Location/Qualifiers
     source          1..532
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     66..84
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(411..439)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 acgtctcatt ttcgccagat atcgacgtct aagaaaccat tattatcatg acattaacct
       61 ataaaaatag gcgtatcacg aggccgatcg gtattcaatt gtgcccaacg ttgccaggta
      121 ttcttgtgat ttatagatcc gcttatagga gaggcttttc ataaaattcc ttttaaaatc
      181 ataacataaa agaatgattc acattaacgg atccgttaac tacgaaaata ggcaacttat
      241 tcttaagggg caagattaat ttatgttttc ccgtcaccaa cgacaaaatt tgcgaggctc
      301 tttccgaaaa tagggttgat ctttgttgtc actggatgta ctgtacatcc atacagtaac
      361 tcacaggggc tggattgatt atgagtaaag gagaagaact tttcactgga gttgtcccaa
      421 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      481 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact gg
//
