LOCUS       sequence-146410-        3861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Pknox2. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Pknox2
FEATURES             Location/Qualifiers
     source          1..3861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(986..1004)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(987..1004)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1022..1038)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1022..1038)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1035..1057)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1046..1062
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1070..1076,1077..1094,1095..1100))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1115..1136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1253..1270)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1424..2012)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1504..1523)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2183..2974,2975..3043))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2806..2825
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3044..3148)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3226..3681)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3363..3384)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3575..3594
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3807..3829
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3821..3838
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3822..3838
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3845..3861,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3845..3861,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tgtgactatc cttttgctcc acccaaccag gtagaggagg agaaggctca gaaggtcgca
      121 agagtgccct ctggttgagc cagatagcca ttcacatcct agcactgaag gagatggcca
      181 atgcacactt acaaatgaag gaactgcaca cccatcacat taaacccaac ggaaaacaca
      241 cgtgtggtca gtcctgtctt tcacagccac agggtgggag gggacaggag gagcagatgc
      301 caagcatggt gtgtgtggat cagctgggcg ctatatcccc tgccatgttg agtcctgaag
      361 gtaagagcag tagcaccttc ttcagaccct ggcttcccat tgggacaatg cttctttcca
      421 atgtttcttc tgccttctct ggagaataga atgaaggagg aggccagaag ctggtagtgc
      481 cagcttggga gcagagggat accacgaaga cctgtggagt ggaaagaacc cctaaaggtt
      541 tatggagggg gctggggtct ctgaagaact ccatctgagg gtaggaacat ctaataagac
      601 actatggtcc tattgtgaca ggagctatgt gatcacgtag ggactgctgg gatccccaaa
      661 gtcatcttaa tatggctcct gatccaggtt ctgctacctg cttggtcctc gagctgaccc
      721 agatgtagag ttgctcttaa cctcttcagg ttggacccac ctacaatgta tttccaagag
      781 aggaagaagg agtctctttg gtttccttcc ccctctcctt atagtccatg gctgctgtgc
      841 caagagacag ggtcctggta gggtaagttg gagccctgtg cagtccacgt tccactctga
      901 tatgaaatca ctagtgaatt cgcggccgcc tgcaggtcga ccatatggga gagctcccaa
      961 cgcgttggat gcatagcttg agtattctat agtgtcacct aaatagcttg gcgtaatcat
     1021 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     1081 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     1141 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     1201 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     1261 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1321 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1381 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1441 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1501 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1561 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1621 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1681 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1741 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1801 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1861 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1921 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1981 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2041 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2101 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2161 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2221 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2281 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2341 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2401 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2461 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2521 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2581 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2641 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2701 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     2761 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     2821 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     2881 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     2941 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3001 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3061 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3121 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgatgcgg
     3181 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta
     3241 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     3301 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     3361 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     3421 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     3481 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     3541 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     3601 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     3661 atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3721 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3781 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3841 attgtaatac gactcactat a
//
