LOCUS       sequence-146412-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AmtR-A1 gate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Nielsen AA, Der BS, Shin J, Vaidyanathan P, Paralanov V, Strychalski
            EA, Ross D, Densmore D, Voigt CA
  TITLE     Genetic circuit design automation.
  JOURNAL   Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
  PUBMED    27034378
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-04-1"; volume: "352(6281):aac7341. doi"; pages: " 
            10.1126/science.aac7341"
COMMENT     Sequence Label: pAmtR-A1
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(74..79,80..95,96..102)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    108..127
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
ORIGIN
        1 tgatgatata tttttatctt gtgcaatgta catcagagat tttgagacac aaggagaacg
       61 atcgttggct gtgttgacaa ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgct
      121 cacaattagg gtgtctcaag gtgcgtacct tgactgatga gtccgaaagg acgaaacacc
      181 cctctacaaa taattttgtt taaaatgttc cctaataatc agcaaagagg ttactagatg
      241 gcaggcgcag ttggtcgtcc gcgtcgtagt gcaccgcgtc gtgcaggtaa aaatccgcgt
      301 gaagaaattc tggatgcaag cgcagaactg tttacccgtc agggttttgc aaccaccagt
      361 acccatcaga ttgcagatgc agttggtatt cgtcaggcaa gcctgtatta tcattttccg
      421 agcaaaaccg aaatctttct gaccctgctg aaaagcaccg ttgaaccgag caccgttctg
      481 gcagaagatc tgagcaccct ggatgcaggt ccggaaatgc gtctgtgggc aattgttgca
      541 agcgaagttc gtctgctgct gagcaccaaa tggaatgttg gtcgtctgta tcagctgccg
      601 attgttggta gcgaagaatt tgcagaatat catagccagc gtgaagcact gaccaatgtt
      661 tttcgtgatc tggcaaccga aattgttggt gatgatccgc gtgcagaact gccgtttcat
      721 attaccatga gcgttattga aatgcgtcgc aatgatggta aaattccgag tccgctgagc
      781 gcagatagcc tgccggaa
//
