LOCUS       sequence-146414-        3949 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Satb1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Satb1
FEATURES             Location/Qualifiers
     source          1..3949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1074..1092)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1075..1092)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1110..1126)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1110..1126)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1123..1145)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1134..1150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1158..1164,1165..1182,1183..1188))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1203..1224
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1341..1358)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1512..2100)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1592..1611)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2271..3062,3063..3131))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2894..2913
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3132..3236)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3314..3769)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3451..3472)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3663..3682
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3895..3917
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3909..3926
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3910..3926
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3933..3949,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3933..3949,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 taggagtgcc ccctttcaca gagcatgatc tcctccattg tgaacagcac gtactatgca
      121 aatgtctcag cagcaaaatg tcaagaattt ggaaggtggt acaaacattt caagaagaca
      181 aaggatatga tggttgagat ggatagtctg tctgaactat cccagcaagg tgccaaccac
      241 gtcaattttg gccagcagcc tgtcccagga aacacagctg agcagcctcc atcccctgcg
      301 cagctctctc acggcagtca gccctctgtc cggacccctc ttccgaacct gcaccctggg
      361 cttgtgtcaa caccgatcag tcctcagctg gtcaaccaac agctggtgat ggctcagttg
      421 ctgaaccagc agtatgcagt gaacagactc ttagcccagc agtccttaaa ccaacagtac
      481 ttgaaccacc ctccccctgt cagtaggtct atgaacaagc ctttggagca gcaagtttcc
      541 acaaacacgg aggtctcttc tgaaatctac cagtgggtgc gggatgaact gaaacgagcc
      601 ggaatctcac aggcagtatt tgcacgcgtg gcttttaacc gaactcaggg attgctttct
      661 gaaatcctcc gaaaggaaga ggaccccaag actgcatccc agtctctgct ggtaaacctt
      721 cgggctatgc agaatttctt acagttgccg gaagccgaaa gagaccggat ataccaggat
      781 gagagggaaa ggagcttgaa cgcagcctca gccatgggtc ctgccccgct gctgagcaca
      841 ccacccagcc gccctcccca ggtgaaaaca gctacccttg ccactgagag aaatgggaag
      901 ccagagaaca atactatgaa cattaatgcc tccatttatg atgagattca gcaggaaatg
      961 aagcgtgcta aagtgtccca agcactgttt gcaaatcact agtgaattcg cggccgcctg
     1021 caggtcgacc atatgggaga gctcccaacg cgttggatgc atagcttgag tattctatag
     1081 tgtcacctaa atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     1141 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     1201 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     1261 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     1321 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1381 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     1441 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1501 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1561 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1621 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1681 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1741 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1801 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1861 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1921 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1981 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2041 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2101 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2161 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2221 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     2281 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     2341 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     2401 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     2461 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     2521 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     2581 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     2641 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     2701 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     2761 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     2821 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     2881 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     2941 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3001 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3061 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3121 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3181 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     3241 tttccccgaa aagtgccacc tgatgcggtg tgaaataccg cacagatgcg taaggagaaa
     3301 ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     3361 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     3421 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     3481 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     3541 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     3601 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     3661 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     3721 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat
     3781 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     3841 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     3901 cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactata
//
