LOCUS       sequence-146416-         479 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Mu-gam under the pBAD promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Cui L, Bikard D
  TITLE     Consequences of Cas9 cleavage in the chromosome of Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 8. pii: gkw223.
  PUBMED    27060147
REFERENCE   2  (bases 1 to 479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 8. pii: gkw223."
COMMENT     Sequence Label: pLC13
FEATURES             Location/Qualifiers
     source          1..479
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg
      301 ctagcgaatt cgagctcggt aactttaaga aggagatata ccatggctaa accagcaaaa
      361 cgtatcaaga gtgccgcagc ggcttatgtg ccacaaaacc gcgatgcggt gattaccgat
      421 attaaacgca tcggggattt acagcgcgaa gcatcacgtc tggaaacgga aatgaatga
//
