LOCUS       sequence-146417-        3868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Six1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Six1
FEATURES             Location/Qualifiers
     source          1..3868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(993..1011)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(994..1011)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1029..1045)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1029..1045)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1042..1064)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1053..1069
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1077..1083,1084..1101,1102..1107))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1122..1143
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1260..1277)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1431..2019)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1511..1530)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2190..2981,2982..3050))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2813..2832
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3051..3155)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3233..3688)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3370..3391)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3582..3601
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3814..3836
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3828..3845
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3829..3845
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3852..3868,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3852..3868,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tctcggtcct tctgctccag agcaatatgg gccacgccag gagctcaaac tattctcttc
      121 caggcctcac agcctcccag cccagccacg gtctgcaagc ccatcagcac cagctccagg
      181 actctctgct gggcccactc acctccagtt tggtggactt gggttcctaa gtggggagat
      241 attggggcct tgaagggatg gccggagcag caaccaaccg cagcaatcta ggggacactt
      301 gtacatagaa gccagggaca attctgcagc ttgtttctgg ggttctttgc gcagaaagga
      361 accacaggct ttcacaattg tctttttaaa aattacaacc aacagcggtc tcagttgagc
      421 atcctctccc tcctctccaa ctcttaatac ccttgcattt cccctcccac taaaaggtca
      481 ggaaataaca aaaacagcca ggcttggttc tgggcaaccc aggcatcctt tacagtagtc
      541 ccttattcca cctcttcttc ctcctcctcc tcgtcttctt ttaatgaaca gaaattacaa
      601 atcagctgga ttgtaattat ttttttaatt tatttatgga gaaacggttt agggaagagg
      661 aaaaggaaat gaagagagat caaaataatg aaaacaagaa accttcagtg tgaggggaac
      721 tggctgcctt cttaagacaa ataggaaaac tataatcaca gctgtttttt gatgggaaga
      781 ttaagtttac atttttcata tttagtgtta aataatttta tgtagattaa aataaaagag
      841 cagtattagc aagggaaaca agtgcctaaa tgtcttaatg ctctctatgg gaggcggtta
      901 gggtagcagc gaaatcacta gtgaattcgc ggccgcctgc aggtcgacca tatgggagag
      961 ctcccaacgc gttggatgca tagcttgagt attctatagt gtcacctaaa tagcttggcg
     1021 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     1081 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     1141 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     1201 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     1261 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1321 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1381 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1441 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1501 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1561 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1621 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1681 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1741 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1801 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1861 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1921 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1981 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2041 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2101 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     2161 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     2221 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     2281 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     2341 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     2401 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     2461 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     2521 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     2581 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     2641 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     2701 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     2761 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     2821 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     2881 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     2941 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3001 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3061 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3121 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     3181 gatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta
     3241 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     3301 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     3361 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     3421 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     3481 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     3541 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     3601 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     3661 gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc aactgttggg
     3721 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     3781 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     3841 ccagtgaatt gtaatacgac tcactata
//
