LOCUS       sequence-146421-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ASB11
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             22..44
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    59..83
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(83..102)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(84..102)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(842..862)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(887..904)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgaggacacg tccatggtat atctccttct taaagttaaa caaaattatt tctagagggg
       61 aattgttatc cgctcacaat tcccctatag tgagtcgtat tacaaaaaac ccctcaagac
      121 ccgtttagag gccccaaggg gggatccgta tccaccttta ctgtcaaaga gcaggtgggc
      181 cttcacggag caagagtgcc tgctccacgc tgctttttgg agccgccaga tcaagcgcac
      241 ttttgccctg agcatttcta cgcttcaggt tagctccata gtcggttagc aggtggatga
      301 cctccacatt ggactgcctc gctgcagcat ggagtggggt gtccagccac tggccatggt
      361 cgacactggc tcctaattct agaagtttct tcacacagtc taccctctgg taggtgcagg
      421 ccacatatag gggagttccg agctgaggca cctcatggtc aatgttaaca ttatttgcca
      481 gcaggatctc catgcactct ctgtgacctc tcttcactgc ctcatggatg ggcgaggcca
      541 ggtgcacctc caactgggcc ttggctccga actccagcag cacattgaca catgcagcac
      601 tgccgctgca gcaagcattg aagaggggtg tggctccgtg aactgtcact ccattgacgt
      661 gtgcaccatt ttccaataag gctttggcac aggccacgtg acctccaagg catgcctcgt
      721 ggagagaaga cacccggtta attgtcacaa ggttcacatt gacaccttgt gcaattaaag
      781 ttttaagggc cagtaagcgc ccctgagctg cagcttcatg aagtggggat cgatcagcca
      841 tggattggaa gtacaggttc tcggtaccca gatctacacc agaagaatga tgatgatgat
      901 ggtgcatatg tatatctc
//
