LOCUS       sequence-146422-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ASB11
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(289..309)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(334..351)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             362..384
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    399..423
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(423..442)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(424..442)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(526..545)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        751..828
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(848..867)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ctgggccttg gctccgaact ccagcagcac attgacacat gcagcactgc cgctgcagca
       61 agcattgaag aggggtgtgg ctccgtgaac tgtcactcca ttgacgtgtg caccattttc
      121 caataaggct ttggcacagg ccacgtgacc tccaaggcat gcctcgtgga gagaagacac
      181 ccggttaatt gtcacaaggt tcacattgac accttgtgca attaaagttt taagggccag
      241 taagcgcccc tgagctgcag cttcatgaag tggggatcga tcagccatgg attggaagta
      301 caggttctcg gtacccagat ctacaccaga agaatgatga tgatgatggt gcatatgtat
      361 atctccttct taaagttaaa caaaattatt tctagagggg aattgttatc cgctcacaat
      421 tcccctatag tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc tacgccggac
      481 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
      541 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
      601 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
      661 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg
      721 agtcgcataa gggagagcgt cgagatcccg gacaccatcg aatggcgcaa aacctttcgc
      781 ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta
      841 acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg
      901 aaccaggcca gc
//
