LOCUS       sequence-146423-        3987 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Tshz1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Tshz1
FEATURES             Location/Qualifiers
     source          1..3987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1112..1130)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1113..1130)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1148..1164)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1148..1164)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1161..1183)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1172..1188
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1196..1202,1203..1220,1221..1226))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1241..1262
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1379..1396)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1550..2138)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1630..1649)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2309..3100,3101..3169))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2932..2951
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3170..3274)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3352..3807)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3489..3510)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3701..3720
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3933..3955
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3947..3964
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3948..3964
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3971..3987,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3971..3987,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tgctggggat gcagagagaa aaatcaaaga ggagactgaa gatgccactg agaagtttga
      121 gcccactgcc ctttatcagt acctgcgtga ggaagaccta gatgacagcc ccaagggagg
      181 agtagatatc ctaaagtcac tcgagaacac tgtgtccacg gccatcagta aagcccagaa
      241 tggggcaccc tcatggggtg ggtaccccag catccatgca gcctaccagc tgccaggcac
      301 agtgaagcca ctgcagtctg ctgtgcagag cgtgcaaata cagccgtctt acgccagcag
      361 cgtgaagtca ctgtcctcca cagagcacaa tgccctccta cactccccag ggagcctcac
      421 cccgccaccc cataagagca atgtgtctgc catggaggag ttagtagaga aggtcacggg
      481 caaggtcagc atcaagaagg aggagaggcc tactgagaag gagaagagct cccctgtcaa
      541 agccatctct cctgtggcaa aggagaataa agatcttcct aagacagagg agaccgggag
      601 caagccacag aagaagggct cggattcaga aactgggaag gccaaaaagg agagtacatt
      661 ggacgctcac accccaaatg gtacagaacc tcttaaagcc aaggtcacca atggctgcgg
      721 ccatctgggt atcatcacgg accactcgcc ggagccttct ttcatcaacc ctctaagcgc
      781 tctgcagtcc atcatgaaca cccacctagg aaaggtgtcc aagcccgtga gcccctctct
      841 ggatccactg gcgatgctct acaagatcag caacagcatg ctggacaagc ccgtctaccc
      901 cacgacccct gccaagcagg ctgatgcaat cgaccgctac tactatgaga acagcgacca
      961 acccattgat ctgaccaagt ctaagaacaa gccactggtc tcgggtgtgg cagatgctgt
     1021 gtcctcgcct caatcactag tgaattcgcg gccgcctgca ggtcgaccat atgggagagc
     1081 tcccaacgcg ttggatgcat agcttgagta ttctatagtg tcacctaaat agcttggcgt
     1141 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     1201 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     1261 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     1321 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     1381 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     1441 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     1501 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     1561 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     1621 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     1681 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1741 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1801 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1861 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     1921 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     1981 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2041 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2101 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2161 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     2221 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     2281 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     2341 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     2401 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     2461 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     2521 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     2581 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     2641 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     2701 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     2761 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     2821 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     2881 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     2941 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     3001 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     3061 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     3121 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     3181 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3241 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     3301 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa
     3361 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     3421 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     3481 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     3541 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     3601 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     3661 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     3721 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     3781 cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga
     3841 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     3901 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     3961 cagtgaattg taatacgact cactata
//
