LOCUS       sequence-146430-        3507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Zfhx3. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Zfhx3
FEATURES             Location/Qualifiers
     source          1..3507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(632..650)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(633..650)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(668..684)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(668..684)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(681..703)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    692..708
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(716..722,723..740,741..746))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    761..782
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(899..916)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1070..1658)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1150..1169)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1829..2620,2621..2689))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2452..2471
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2690..2794)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2872..3327)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3009..3030)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3221..3240
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3453..3475
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3467..3484
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3468..3484
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3491..3507,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3491..3507,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tcagcttctt ctggtgtttg atgaggtcaa agatgcgctg aaacacgagg ctgcactttt
      121 tgcactggta attcaagttg ctcgtccgaa tgtagcgatc gttggtaagc tcgcgccgct
      181 ctccgtcttt gccctccccc tgattctcgt aattcttcct ggctttctgt cgggcgttct
      241 gaaaccacac cactatgaca cgggttggaa ggttcagtaa gttagaaagc tgctcaaatt
      301 catcatcctt tgggtaagca ttggcatcaa agaagtcttg caggactcgg agctggtagt
      361 cggtaaacct tgttctcgag gaccgcttgc tcccccagta ctcctgcttc tggggttcgg
      421 gggaaggggg ccgggagtcg atcttgagtt cctccagact ggtgatggga gggttgctga
      481 agttgtacgg ggagtccttg ttccgttgcc gctccttgaa tagagtgttt ctgaaccagt
      541 gcttgatcac taatcactag tgaattcgcg gccgcctgca ggtcgaccat atgggagagc
      601 tcccaacgcg ttggatgcat agcttgagta ttctatagtg tcacctaaat agcttggcgt
      661 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
      721 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
      781 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
      841 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
      901 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      961 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     1021 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     1081 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     1141 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     1201 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1261 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1321 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1381 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     1441 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     1501 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     1561 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1621 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1681 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     1741 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     1801 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     1861 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     1921 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     1981 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     2041 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     2101 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     2161 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     2221 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     2281 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     2341 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     2401 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     2461 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     2521 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     2581 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     2641 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     2701 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     2761 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     2821 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa
     2881 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2941 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     3001 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     3061 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     3121 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     3181 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     3241 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     3301 cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga
     3361 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     3421 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     3481 cagtgaattg taatacgact cactata
//
