LOCUS       sequence-146431-        3837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Zhx1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Zhx1
FEATURES             Location/Qualifiers
     source          1..3837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(962..980)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(963..980)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(998..1014)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(998..1014)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1011..1033)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1022..1038
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1046..1052,1053..1070,1071..1076))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1091..1112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1229..1246)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1400..1988)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1480..1499)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2159..2950,2951..3019))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2782..2801
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3020..3124)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3202..3657)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3339..3360)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3551..3570
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3783..3805
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3797..3814
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3798..3814
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3821..3837,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3821..3837,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tagagccaag tgcctgctgc tcagcctgct acagacacaa aaccagccac agcagcagtt
      121 ccatcctcgc caagtgtcag acctgaagct gcactagtga acccagattc atttggtatt
      181 cgggccaaaa agactaaaga gcagctggca gaactgaaag ttagctacct aaaaaatcag
      241 tttccccatg actcagagat catcaggctt atgaaaataa caggccttac caaaggcgag
      301 attaaaaaat ggtttagtga tacaaggtac aaccagagaa attcaaagag taaccagtgc
      361 ttacatctca acaatgactc ctctgccact atcatcatag actccagtga tgaaacccca
      421 gaacccccag cggcagctgc ttcacagcag aaacagtcct ggaatccctt tcctgacttt
      481 gctccccaga agtttaaaga gaaaactgca gagcaactcc gtgcccttca ggcaagtttt
      541 ctcaacagtt ctgtacttac tgatgaagaa gtaaataggt taagagcgca aaccaaactt
      601 actagaagag aaattgatgc ttggtttaca gagaaaaata aaacaaaagc tttaaaggat
      661 gagaaaatag aagtagatga aagtaatgta ggtagttcca aagaagagcc tggagaaagt
      721 tctcctggag atgaaacagt tgcacctaag tcaggaggga caggcaagat atgtaagaaa
      781 acaccagagc agctgcacat gcttaaaagt gcatttgtcc gaacacagtg gccatcagca
      841 gaagagtatg acaagctggc tgaagagagt ggccttgcca gaatcactag tgaattcgcg
      901 gccgcctgca ggtcgaccat atgggagagc tcccaacgcg ttggatgcat agcttgagta
      961 ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     1021 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     1081 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     1141 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     1201 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1261 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1321 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1381 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1441 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1501 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1561 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1621 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1681 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1741 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1801 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1861 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1921 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1981 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2041 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2101 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2161 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     2221 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2281 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     2341 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2401 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2461 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2521 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2581 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2641 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2701 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2761 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     2821 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     2881 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     2941 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3001 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3061 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3121 cgcgcacatt tccccgaaaa gtgccacctg atgcggtgtg aaataccgca cagatgcgta
     3181 aggagaaaat accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     3241 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     3301 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     3361 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     3421 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     3481 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     3541 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     3601 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca
     3661 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     3721 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     3781 ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact cactata
//
