LOCUS       sequence-146432-        3825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Zhx2. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Zhx2
FEATURES             Location/Qualifiers
     source          1..3825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(950..968)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(951..968)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(986..1002)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(986..1002)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(999..1021)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1010..1026
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1034..1040,1041..1058,1059..1064))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1079..1100
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1217..1234)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1388..1976)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1468..1487)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2147..2938,2939..3007))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2770..2789
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3008..3112)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3190..3645)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3327..3348)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3539..3558
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3771..3793
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3785..3802
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3786..3802
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3809..3825,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3809..3825,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 taccaaggga agccaaaggc atttgagagg gagtagagcc aaaagctgag aatctcactg
      121 tcctgcaccc acccacccac tccagaaaga agctgttgct cagaggcaga cggcatggca
      181 agcaaacgga aatctacaac tccctgcatg gttcggacat cacaggtact ggaacaagat
      241 atgctggagg aggcagacag ggccaaagac aaaggagcgg gcatgccaca gtccgatgtg
      301 acaaaggaca gctgggcagc ggaacctgaa cattcgtcca aagaaaccga agtggttgag
      361 gtgaaatcta tgggggagaa cctgtccaaa aaactacagg gcggttatga gtgcaaatac
      421 tgcccttatt ccacgcaaaa tctgaatgag ttcacagaac acgtggacat gcaacatcct
      481 aacgtgattc tcaaccccct ctacgtatgt gccgaatgta acttcacaac caaaaagtac
      541 gactccttgt ctgaccacaa ctccaagttc catccggggg agaccaactt caagctgaaa
      601 ttaatcaagc gcaataatca gacggttcta gaacagtcca tcgaagccac caaccacgtc
      661 gtgcccatca ctgccagtgg tcctggaagt agtgataacg accctggggt ctcggtagga
      721 aaaaccccca tgacaaagac aggaaagctg aaggcagatg ccaagaaagt gcccaagaag
      781 cctgatgagg ctgccccaga aaaccacatg gaagggaccg cccgcctggt gacagacaca
      841 gctgagatcc tcgccagact tggtagcgta atcactagtg aattcgcggc cgcctgcagg
      901 tcgaccatat gggagagctc ccaacgcgtt ggatgcatag cttgagtatt ctatagtgtc
      961 acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     1021 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     1081 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     1141 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     1201 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     1261 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     1321 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1381 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1441 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1501 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1561 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1621 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1681 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1741 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1801 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1861 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1921 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1981 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2041 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     2101 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     2161 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     2221 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     2281 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2341 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     2401 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     2461 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2521 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     2581 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     2641 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2701 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2761 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     2821 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     2881 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     2941 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3001 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3061 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3121 cccgaaaagt gccacctgat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     3181 cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     3241 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     3301 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     3361 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     3421 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     3481 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     3541 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     3601 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag
     3661 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     3721 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     3781 acgttgtaaa acgacggcca gtgaattgta atacgactca ctata
//
