LOCUS       sequence-146433-        3618 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Zhx3. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Zhx3
FEATURES             Location/Qualifiers
     source          1..3618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(743..761)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(744..761)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(779..795)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(779..795)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(792..814)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    803..819
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(827..833,834..851,852..857))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    872..893
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1010..1027)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1181..1769)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1261..1280)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1940..2731,2732..2800))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2563..2582
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2801..2905)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2983..3438)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3120..3141)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3332..3351
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3564..3586
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3578..3595
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3579..3595
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3602..3618,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3602..3618,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 ttgaaggtaa cagtgggctc tttgtcaatc agaacacaag acaagtctgg cacaaaatgt
      121 ggcttgcctc cactcagtga tgcagagatc tcttccaaca atctgttccc ttgagaagac
      181 aagtagggcc aggcctggtg cagttctgag catgcctggg actcccactg ttgcagagga
      241 tgagggtccc tgtggtctca gcttcccaga aaacagacat ggcattgtca gagctgtact
      301 gttttggtaa cccgagttcc tatgtattga aaatgtctgt agtagtatgc tttattaaat
      361 gaatataggc agctgtatat gctaacaact tgattctggg tctaggtata tgcaatctga
      421 attgtccctg ggaggccatg gaaaggtcct gctctgtggg aagatgggct gatgagaaac
      481 cattgctatt tgctcagaaa tgtcaggcgg aaggtggttg gggcggtggc aggttctggg
      541 ttcagcgagg ctccagaggc tctcctggtg cctggcagct ggccatcaag atgatatgtg
      601 gcagttttgg cccttccaca tggcatacag catcccagac tggccggtcc ttcacagtaa
      661 tcaatcacta gtgaattcgc ggccgcctgc aggtcgacca tatgggagag ctcccaacgc
      721 gttggatgca tagcttgagt attctatagt gtcacctaaa tagcttggcg taatcatggt
      781 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      841 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
      901 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
      961 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     1021 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     1081 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     1141 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1201 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1261 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1321 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1381 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1441 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1501 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1561 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1621 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1681 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1741 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1801 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     1861 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1921 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     1981 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2041 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     2101 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     2161 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     2221 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     2281 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     2341 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     2401 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     2461 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     2521 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     2581 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     2641 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     2701 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     2761 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     2821 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     2881 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gatgcggtgt
     2941 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta agcgttaata
     3001 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     3061 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     3121 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     3181 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     3241 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     3301 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     3361 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     3421 cgccgctaca gggcgcgtcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     3481 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     3541 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
     3601 gtaatacgac tcactata
//
