LOCUS       sequence-146435-        3718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Neurog1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Chang I, Parrilla M
  TITLE     Expression patterns of homeobox genes in the mouse vomeronasal organ
            at postnatal stages.
  JOURNAL   Gene Expr Patterns. 2016 Jul;21(2):69-80. doi: 
            10.1016/j.gep.2016.08.001. Epub 2016 Aug 9.
  PUBMED    27521061
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.gep.2016.08.001"; journalName: "Gene Expr Patterns"; 
            date: "2016-07"; volume: "21"; issue: "2"; pages: "69-80"
COMMENT     Sequence Label: pISH-Neurog1
FEATURES             Location/Qualifiers
     source          1..3718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(843..861)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(844..861)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(879..895)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(879..895)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(892..914)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    903..919
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(927..933,934..951,952..957))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    972..993
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1110..1127)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1281..1869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1361..1380)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2040..2831,2832..2900))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2663..2682
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2901..3005)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3083..3538)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3220..3241)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3432..3451
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3664..3686
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3678..3695
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3679..3695
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3702..3718,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3702..3718,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tcgtcgtgtg gagcaggtct ttgggcaggc caggaaagga gaaaagggga tcgcccgggc
      121 cataggtgaa gtcttctgaa gccgagggac tactggggtc agagagtggt gatgccacag
      181 tggcgcaggg ggaggcagcg gccccggaac cccaggactc agtgtcgctg gccgggctcg
      241 ggggcccggg cagacagggg acacactgcg gaggcaggag gcgctcccgg gcactgcccc
      301 cggggagccc ttgatctgcc aggcgcagtg tctcagccag ggcccagatg tagttgtagg
      361 cgaagcgcag cgtctcaatc ttggtgagct tggtgtcgtc ggggaacgag ggcagcacgc
      421 tgcgcaaggc gtccagcgca gcgttgaggt tgtgcatgcg gttgcgctcg cgatcgttgg
      481 ctttgacgcg acgactcctc cgcagggagt gcagcagagc ctcggaccgc acccgagcgc
      541 gacctcgccg cctccgccgt tcctgctctt cgtcctgggc accgggaaca ttcgatgccc
      601 cggagagggc gggagcgctc ctccgggctg gcacggacag ccccgaggtg gaggctaggg
      661 gctgtagcct ggcacagtcc tcctcgtcgg tgaggaagct ggacaggtcg ctgctgctgt
      721 tgctgctgga gcagtcgaga tcagagatgc aggtctccaa agaatcacta gtgaattcgc
      781 ggccgcctgc aggtcgacca tatgggagag ctcccaacgc gttggatgca tagcttgagt
      841 attctatagt gtcacctaaa tagcttggcg taatcatggt catagctgtt tcctgtgtga
      901 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
      961 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     1021 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     1081 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1141 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1201 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1261 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1321 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1381 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1441 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1501 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1561 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1621 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1681 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1741 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1801 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1861 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1921 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1981 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     2041 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     2101 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     2161 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     2221 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     2281 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     2341 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     2401 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     2461 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     2521 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     2581 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     2641 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     2701 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     2761 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     2821 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     2881 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     2941 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     3001 ccgcgcacat ttccccgaaa agtgccacct gatgcggtgt gaaataccgc acagatgcgt
     3061 aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta
     3121 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     3181 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     3241 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     3301 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     3361 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     3421 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     3481 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     3541 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     3601 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     3661 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactata
//
