LOCUS       sequence-146436-        3939 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Neurod1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Chang I, Parrilla M
  TITLE     Expression patterns of homeobox genes in the mouse vomeronasal organ
            at postnatal stages.
  JOURNAL   Gene Expr Patterns. 2016 Jul;21(2):69-80. doi: 
            10.1016/j.gep.2016.08.001. Epub 2016 Aug 9.
  PUBMED    27521061
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.gep.2016.08.001"; journalName: "Gene Expr Patterns"; 
            date: "2016-07"; volume: "21"; issue: "2"; pages: "69-80"
COMMENT     Sequence Label: pISH-Neurod1
FEATURES             Location/Qualifiers
     source          1..3939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1064..1082)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1065..1082)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1100..1116)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1100..1116)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1113..1135)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1124..1140
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1148..1154,1155..1172,1173..1178))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1193..1214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1331..1348)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1502..2090)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1582..1601)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2261..3052,3053..3121))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2884..2903
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3122..3226)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3304..3759)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3441..3462)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3653..3672
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3885..3907
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3899..3916
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3900..3916
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3923..3939,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3923..3939,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tctgactgca ccagcccttc ctttgacgga cccctcagcc cgccgctcag catcaatggc
      121 aacttctctt tcaaacacga accatccgcc gagtttgaaa aaaattatgc ctttaccatg
      181 cactaccctg cagcgacgct ggcagggccc caaagccacg gatcaatctt ctcttccggt
      241 gccgctgccc ctcgctgcga gatccccata gacaacatta tgtctttcga tagccattcg
      301 catcatgagc gagtcatgag tgcccagctt aatgccatct ttcacgatta gaggcacgtc
      361 agtttcacta ttcccgggaa acgaatccac tgtgcgtaca gtgactgtcc tgtttacaga
      421 aggcagccct tttgctaaga ttgctgcaaa gtgcaaatac tcaaagcttc aagtgatata
      481 tgtatttatt gtcgttactg cctttggaag aaacagggga tcaaagttcc tgttcacctt
      541 atgtattgtt ttctatagct cttctgtttt aaaaataata atacagtaaa gtaaaaaaga
      601 aaatgtgtac cacgaatttc gtgtagctgt attcagatcg tattaattat ctgatcggga
      661 taaaaaaaaa tcacaagcaa taattaggat ctatgcaatt tttaaactag taatgggcca
      721 attaaaatat atataaatat atatttttca accagcattt tactacctgt gacctttccc
      781 atgctgaatt attttgttgt gattttgtac agaattttta atgacttttt ataacgtgga
      841 tttcctattt taaaaccatg cagcttcatc aatttttata catatcagaa aagtagaatt
      901 atatctaatt tatacaaaat aatttaacta atttaaacca gcagaaaagt gcttagaaag
      961 ttattgcgtt gccttagcac ttcaatcact agtgaattcg cggccgcctg caggtcgacc
     1021 atatgggaga gctcccaacg cgttggatgc atagcttgag tattctatag tgtcacctaa
     1081 atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     1141 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     1201 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     1261 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     1321 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1381 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1441 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1501 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1561 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1621 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1681 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1741 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1801 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1861 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1921 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1981 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2041 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2101 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2161 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2221 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     2281 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2341 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     2401 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     2461 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     2521 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2581 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2641 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     2701 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     2761 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     2821 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     2881 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     2941 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3001 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3061 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3121 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     3181 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     3241 aagtgccacc tgatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     3301 aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     3361 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     3421 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     3481 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     3541 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     3601 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     3661 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     3721 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg
     3781 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     3841 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     3901 taaaacgacg gccagtgaat tgtaatacga ctcactata
//
