LOCUS       sequence-146440-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Escherichia coli glycinamide ribonucleotide synthetase 
            (H6EcPurD).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Kappock
  TITLE     Purine biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDC10
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 aacagcggcc tggtgccgcg cggcagcgaa ttcatgaaag tattagtgat tggtaacggc
      121 gggcgcgagc acgcgctggc ctggaaagcg gcccagtcgc cgctggttga gactgttttt
      181 gttgctccgg gtaatgcagg cactgcactg gaacccgcgc tgcaaaacgt tgctattggc
      241 gtgaccgata tcccggcgct gttggatttc gcacaaaacg aaaagattga tctgaccatc
      301 gtcggcccgg aagcgccgct ggtgaaaggc gtggtcgata ccttccgcgc cgccgggctg
      361 aaaatcttcg gcccaaccgc gggtgcggcc caactggaag gctcaaaagc gtttaccaaa
      421 gatttcctgg cccgccataa gatccctacg gcggaatacc agaacttcac cgaggtagaa
      481 cctgcgctgg cgtatctgcg tgagaaaggc gcgccaatcg tcattaaagc ggacggtctg
      541 gctgccggga aaggcgttat cgtggcgatg acgctggaag aagcggaagc ggctgttcac
      601 gatatgctgg cgggcaacgc ttttggcgac gcgggtcatc gcatcgttat cgaagagttc
      661 ctcgatggcg aagaagcgag ctttatcgtg atggtggacg gcgagcatgt gctgccgatg
      721 gctaccagcc aggatcacaa acgcgtaggc gataaagata ccggaccaaa caccggcggg
      781 atgggcgctt actcccccgc gccggtagta accgatgacg ttcatcagcg caccatggaa
      841 cgtattatct ggccaaccgt gaaaggcatg gcggcggaag gcaacacct
//
