LOCUS       sequence-146453-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lenti sgRNA cloning backbone with MS2 loops at tetraloop and 
            stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI 
            sites for insertion of spacer sequences..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham
            MD, Sanjana NE, Zhang F
  TITLE     Genome-scale CRISPR-Cas9 knockout and transcriptional activation 
            screening.
  JOURNAL   Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016. 
            Epub 2017 Mar 23.
  PUBMED    28333914
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2017.016"; journalName: "Nat Protoc"; date: 
            "2017-04"; volume: "12"; issue: "4"; pages: "828-863"
COMMENT     Sequence Label: lentiSAMv2
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cagttcgctc aggccgcctc tctcggcctt ggtcagattg tcgaactttc tctgggtaat
       61 cagcttggcg ttcagcagct gccgccagta gttcttcatc ttcttcacga cctcttcgga
      121 gggcacgttg tcgctcttgc cccgggcctt gtcgcttctg gtcagcacct tgttgtcgat
      181 ggagtcgtcc ttcagaaagc tctgaggcac gatgtggtcc acatcgtagt cggacagccg
      241 gttgatgtcc agttcctggt ccacgtacat atcccgccca ttctgcaggt agtacaggta
      301 cagcttctcg ttctgcagct gggtgttttc cacggggtgt tctttcagga tctggctgcc
      361 cagctctttg atgccctctt cgatccgctt cattctctcg cggctgttct tctgtccctt
      421 ctgggtggtc tggttctctc tggccatttc gatcacgatg ttctcgggct tgtgccggcc
      481 catcactttc acgagctcgt ccaccacctt cactgtctgc aggatgccct tcttaatggc
      541 ggggctgccg gccagattgg caatgtgctc gtgcaggcta tcgccctggc cggacacctg
      601 ggctttctgg atgtcctctt taaaggtcag gctgtcgtcg tggatcagct gcatgaagtt
      661 tctgttggcg aagccgtcgg acttcaggaa atccaggatt gtcttgccgg actgcttgtc
      721 ccggatgccg ttgatcagct tccggctcag cctgccccag ccggtgtatc tccgccgctt
      781 cagctgcttc atcactttgt cgtcgaacag gtgggcatag gttttcagcc gttcctcgat
      841 catctctctg tcctcaaaca gtgtcagggt cagcacgata tcttccagaa tgtcctcgtt
      901 ttcctcattg tccaggaagt ccttgtcctt gataatttt
//
