LOCUS       sequence-146458-        7332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Trans plasmid for production of recombinant AAV-B1 vectors. Plasmid 
            backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep 
            and AAV-B1 capsid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7332)
  AUTHORS   Choudhury SR, Fitzpatrick Z, Harris AF, Maitland SA, Ferreira JS, 
            Zhang Y, Ma S, Sharma RB, Gray-Edwards HL, Johnson JA, Johnson AK, 
            Alonso LC, Punzo C, Wagner KR, Maguire CA, Kotin RM, Martin DR, 
            Sena-Esteves M
  TITLE     In vivo selection yields AAV-B1 capsid for CNS and muscle gene 
            therapy.
  JOURNAL   Mol Ther. 2016 Apr 27. doi: 10.1038/mt.2016.84.
  PUBMED    27117222
REFERENCE   2  (bases 1 to 7332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2016 Apr 27. doi: 10.1038/mt.2016.84."
COMMENT     Sequence Label: pGG-B1
FEATURES             Location/Qualifiers
     source          1..7332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3938..3949
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(4400..4420)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4400..4418)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4439..4455)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4439..4455)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4452..4474)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4463..4479
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4487..4493,4494..4511,4512..4517))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4532..4553
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4670..4687)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4841..5429)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4921..4940)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5600..6391,6392..6460))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6223..6242
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6461..6565)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6591..7046)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6728..6749)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6940..6959
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7173..7195
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7187..7204
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7188..7204
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7214..7233
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7214..7232
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     7261..7277
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7262..7278
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atgccggggt tttacgagat tgtgattaag gtccccagcg accttgacga gcatctgccc
       61 ggcatttctg acagctttgt gaactgggtg gccgagaagg aatgggagtt gccgccagat
      121 tctgacatgg atctgaatct gattgagcag gcacccctga ccgtggccga gaagctgcag
      181 cgcgactttc tgacggaatg gcgccgtgtg agtaaggccc cggaggctct tttctttgtg
      241 caatttgaga agggagagag ctacttccac atgcacgtgc tcgtggaaac caccggggtg
      301 aaatccatgg ttttgggacg tttcctgagt cagattcgcg aaaaactgat tcagagaatt
      361 taccgcggga tcgagccgac tttgccaaac tggttcgcgg tcacaaagac cagaaatggc
      421 gccggaggcg ggaacaaggt ggtggatgag tgctacatcc ccaattactt gctccccaaa
      481 acccagcctg agctccagtg ggcgtggact aatatggaac agtatttaag cgcctgtttg
      541 aatctcacgg agcgtaaacg gttggtggcg cagcatctga cgcacgtgtc gcagacgcag
      601 gagcagaaca aagagaatca gaatcccaat tctgatgcgc cggtgatcag atcaaaaact
      661 tcagccaggt acatggagct ggtcgggtgg ctcgtggaca aggggattac ctcggagaag
      721 cagtggatcc aggaggacca ggcctcatac atctccttca atgcggcctc caactcgcgg
      781 tcccaaatca aggctgcctt ggacaatgcg ggaaagatta tgagcctgac taaaaccgcc
      841 cccgactacc tggtgggcca gcagcccgtg gaggacattt ccagcaatcg gatttataaa
      901 attttggaac taaacgggta cgatccccaa tatgcggctt ccgtctttct gggatgggcc
      961 acgaaaaagt tcggcaagag gaacaccatc tggctgtttg ggcctgcaac taccgggaag
     1021 accaacatcg cggaggccat agcccacact gtgcccttct acgggtgcgt aaactggacc
     1081 aatgagaact ttcccttcaa cgactgtgtc gacaagatgg tgatctggtg ggaggagggg
     1141 aagatgaccg ccaaggtcgt ggagtcggcc aaagccattc tcggaggaag caaggtgcgc
     1201 gtggaccaga aatgcaagtc ctcggcccag atagacccga ctcccgtgat cgtcacctcc
     1261 aacaccaaca tgtgcgccgt gattgacggg aactcaacga ccttcgaaca ccagcagccg
     1321 ttgcaagacc ggatgttcaa atttgaactc acccgccgtc tggatcatga ctttgggaag
     1381 gtcaccaagc aggaagtcaa agactttttc cggtgggcaa aggatcacgt ggttgaggtg
     1441 gagcatgaat tctacgtcaa aaagggtgga gccaagaaaa gacccgcccc cagtgacgca
     1501 gatataagtg agcccaaacg ggtgcgcgag tcagttgcgc agccatcgac gtcagacgcg
     1561 gaagcttcga tcaactacgc ggacaggtac caaaacaaat gttctcgtca cgtgggcatg
     1621 aatctgatgc tgtttccctg cagacaatgc gagagactga atcagaattc aaatatctgc
     1681 ttcactcacg gtgtcaaaga ctgtttagag tgctttcccg tgtcagaatc tcaacccgtt
     1741 tctgtcgtca aaaaggcgta tcagaaactg tgctacattc atcacatcat gggaaaggtg
     1801 ccagacgctt gcactgcttg cgacctggtc aatgtggact tggatgactg tgtttctgaa
     1861 caataaatga cttaaaccag gtatggctgc cgatggttat cttccagatt ggctcgagga
     1921 caacctctct gagggcattc gcgagtggtg ggacttgaaa cctggagccc cgaagcccaa
     1981 agccaaccag caaaagcagg acgacggccg gggtctggtg cttcctggct acaagtacct
     2041 cggacccttc aacggactcg acaaggggga gcccgtcaac gcggcggacg cagcggccct
     2101 cgagcacgac aaggcctacg accagcagct caaagcgggt gacaatccgt acctgcggta
     2161 taaccacgcc gacgtcgagt ttcaggagcg tctgcaagaa gatacgtctt ttgggggcaa
     2221 cctcgggcga gcagtcttcc aggccaagaa gagggttctc gaaccttttg gtctggttga
     2281 ggaaggcgct aagacggctc ctggaaagaa acgtccggta gagcagtcgc cacaagagcc
     2341 agactcctcc tcgggcatcg gcaagaaagg ccaacagccc gccagaaaaa gactcaattt
     2401 tggccagact ggcgactcan agtcagttcc agaccctcaa cctctcggag aacctccagc
     2461 agcgccctct ggtgtgggac ctaatacaat ggctgcaggc ggtggcgcac caatggcaga
     2521 caataacgag ggcgccgacg gagtgngtaa ttcctcggga aattggcatt gcgattccac
     2581 atggatgggg gacagagtca tcaccaccag cacccgaacc tgggccctgc ccacctacaa
     2641 caaccacctc tacaagcaaa tctccaacgg cacctcggga ggaagcacca acgacaacac
     2701 ctattttggc tacagcaccc cctgggggta ttttgatttc aacagattcc actgccactt
     2761 ctcaccacgt gactggcagc gactcatcaa caacaactgg ggattccggc caaaaagact
     2821 cagcttcaag ctcttcaaca tccaggtcaa ggaggtcacg cagaatgaag gcaccaagac
     2881 catcgccaat aaccttacca gcacgattca ggtatttacg gactcggaat accagctgcc
     2941 atacgtcctc ggctccgcgc accagggctg cctgcctccg ttcccggcgg acgtcttcat
     3001 gattccccag tacggctacc ttacactgaa caatggaagt caagccgtag gccgttcctc
     3061 cttctactgc ctggaatatt ttccttctca aatgctgaga acgggcaaca actttgagtt
     3121 cagctaccag tttgaggacg tgccttttca cagcagctac gcgcacagcc aaagcctgga
     3181 ccggctgatg aaccccctca tcgaccagta cctgtactac ctgtctcgga ctcagtccac
     3241 gggaggtacc gcaagaactc agcagttgct attttctcag gccgggccta atacaatggc
     3301 caatcaggca aagaactggc tgccaggacc ctgttaccgc caacaacgcg tctcaacgac
     3361 aaccgggcaa aacaacaata gcaactctgc ctggactgct gggaccaaat accatctgaa
     3421 tggaagaaat tcattggcta atcctggcat cgctatggca acacacaaag acgacgagga
     3481 gcgttttttt cccagtaacg ggatcctgat ttttggcaaa caaaatgctg ccagagacaa
     3541 tgcggattac agcgatgtca tgctcaccag cgaggaagaa atcaaaacca ctaaccctgt
     3601 ggctacagag gaatacggta tcgtggcaga taacttgcag cagcaaaaca cggctcctca
     3661 aattggaact gtcaacagcc agggggcctt acccggtatg gtctggcaga accgggacgt
     3721 gtacctgcag ggtcccatct gggccaagat tcctcacacg gacggcaact tccacccatc
     3781 tccgctgatg ggcggctttg gcctgaaaca tcctccgcct cagatcctga tcaagaacac
     3841 gcctgtacct gcggatcctc cgaccacctt caaccagtca aagctgaact ctttcatcac
     3901 gcaatacagc accggacagg tcagcgtgga aattgaatgg gagctacaga aggaaaacag
     3961 caagcgctgg aaccccgaga tccagtacac ctccaactac tacaaatcta caagtgtgga
     4021 ctttgctgtt aatacagaag gcgtgtactc tgaaccccgc cccattggca cccgttacct
     4081 cacccgtaat ctgtaattgc ttgttaatca ataaaccgtt taattcgttt cagttgaact
     4141 ttggtctctg cgaagggcga attcgtttaa acctgcagga ctagagtcct gtattagagg
     4201 tcacgtgagt gttttgcgac attttgcgac accatgtggt cacgctgggt atttaagccc
     4261 gagtgagcac gcagggtctc cattttgaag cgggaggttt gaacgcgcag ccgccaagcc
     4321 gaattctgca gatatccatc acactggcgg ccgctcgact agagcggccg ccaccgcggt
     4381 ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
     4441 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     4501 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     4561 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     4621 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     4681 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     4741 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     4801 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     4861 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     4921 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     4981 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5041 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5101 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5161 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5221 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5281 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5341 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5401 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5461 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5521 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5581 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5641 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     5701 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     5761 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     5821 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     5881 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     5941 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6001 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6061 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6121 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6181 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6241 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6301 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6361 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6421 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6481 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6541 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
     6601 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     6661 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     6721 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     6781 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     6841 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     6901 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     6961 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     7021 gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga
     7081 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     7141 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     7201 cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtaccgggc cccccctcga
     7261 tcgaggtcga cggtatcggg ggagctcgca gggtctccat tttgaagcgg gaggtttgaa
     7321 cgcgcagccg cc
//
