LOCUS       sequence-146463-        1767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent mitophagy reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Rojansky R, Cha MY, Chan DC
  TITLE     Elimination of paternal mitochondria in mouse embryos occurs through
            autophagic degradation dependent on PARKIN and MUL1.
  JOURNAL   Elife. 2016 Nov 17;5. pii: e17896. doi: 10.7554/eLife.17896.
  PUBMED    27852436
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-11-17"; volume: "5. pii: e17896. doi"; pages: " 
            10.7554/eLife.17896"
COMMENT     Sequence Label: pCLBW cox8 EGFP mCherry
FEATURES             Location/Qualifiers
     source          1..1767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    86..110
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             117..191
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             join(213..215,216..926)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(213..215,216..926)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(255..276)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(516..535)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     863..884
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             942..1652
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1087..1105)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1355..1374
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(1653..1677)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctcngngcac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tgatttgata
       61 ccgcgggccc gggatccctc gaatcacaag tttgtacaaa aaagcaggct gacaccatgt
      121 ccgtcctgac gccgctgctg ctgcggggct tgacaggctc ggcccggcgg ctcccagtgc
      181 cgcgcgccaa gatccattcg ttgggggatc cggtgagcaa gggcgaggag ctgttcaccg
      241 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      301 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      361 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
      421 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      481 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      541 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      601 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
      661 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
      721 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
      781 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
      841 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
      901 ctctcggcat ggacgagctg tacaagggct caggctcggg catggtgagc aagggcgagg
      961 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     1021 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     1081 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     1141 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     1201 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     1261 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     1321 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     1381 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     1441 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     1501 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     1561 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     1621 ccaccggcgg catggacgag ctgtataagt aaacccagct ttcttgtaca aagtggtgat
     1681 tcgaggccgc gactctagaa ttcgatatca agcttatcga taatcaacct ctggattaca
     1741 aaatttgnga aagangactg gtatnna
//
