LOCUS       sequence-146469-        3987 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Stmn2. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Chang I, Parrilla M
  TITLE     Expression patterns of homeobox genes in the mouse vomeronasal organ
            at postnatal stages.
  JOURNAL   Gene Expr Patterns. 2016 Jul;21(2):69-80. doi: 
            10.1016/j.gep.2016.08.001. Epub 2016 Aug 9.
  PUBMED    27521061
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.gep.2016.08.001"; journalName: "Gene Expr Patterns"; 
            date: "2016-07"; volume: "21"; issue: "2"; pages: "69-80"
COMMENT     Sequence Label: pISH-Stmn2
FEATURES             Location/Qualifiers
     source          1..3987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1112..1130)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1113..1130)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1148..1164)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1148..1164)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1161..1183)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1172..1188
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1196..1202,1203..1220,1221..1226))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1241..1262
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1379..1396)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1550..2138)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1630..1649)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2309..3100,3101..3169))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2932..2951
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3170..3274)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3352..3807)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3489..3510)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3701..3720
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3933..3955
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3947..3964
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3948..3964
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3971..3987,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3971..3987,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tcaggttgaa ctgtctggct gaagcaatgg cgggtatggc aagcccctcc catagtaaat
      121 ccccctgcct atattataat ggatcatgcg atatcagtat gggaagtgta tgacatggtt
      181 taaaaagaac tcattatgaa aaacaaaaca aaaaacagaa tcaaaaataa aataaaaatc
      241 aatgcggtct ctttgcagaa tgttttgctt gatgtttaaa aaagtacctt ggatcttatt
      301 ttgtaaatat ttacattttt gttaaaaaat acaagtattg cattatgcaa gttatttcat
      361 gatcttacat gtcctgtaac aggcttttga cgttgtgtct ttccactcag atggatttgc
      421 taggtctgtt cttttggaag ccccactcag tccctgccct tttcattcca accgaaaaat
      481 gaggtcagta gacagtctat ggtgctagaa acccaccatt gcctaatgac ctagatgact
      541 ccgtaatctc tgagcgtgac caagaccaca cctagatcgt gggtactgtg tgtccatgtg
      601 aacctccaca tttgctcgta atctgcttcc tgcctagaag cggcaaagcc aggctaaggg
      661 aatctaccag tcatagcact tatgtctgct cctcatggat tacgcgctac acatgtgttt
      721 gggttttaga aacaggtgca tgtgaagaca aatctgagct cagtcagggt agaagcgaaa
      781 ggctgaacct tccaacgtgc tgatcacaaa cgcaaagcca gagggttcaa gcagccatgg
      841 gattgttctt catgaagtgg gcttatttct ctcattttag ttattatgat gggatgaatt
      901 aattgacatg tcgggtggct tatctgtggg tgccatgggt gatgatgttc attttaagct
      961 tttgcctgta gtacaagcac ataatgctac tgaacgtttt tccacttgga gactgtgagc
     1021 tggttgctgc aaatcactag tgaattcgcg gccgcctgca ggtcgaccat atgggagagc
     1081 tcccaacgcg ttggatgcat agcttgagta ttctatagtg tcacctaaat agcttggcgt
     1141 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     1201 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     1261 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     1321 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     1381 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     1441 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     1501 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     1561 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     1621 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     1681 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1741 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1801 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1861 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     1921 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     1981 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2041 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2101 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2161 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     2221 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     2281 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     2341 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     2401 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     2461 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     2521 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     2581 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     2641 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     2701 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     2761 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     2821 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     2881 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     2941 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     3001 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     3061 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     3121 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     3181 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3241 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     3301 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa
     3361 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     3421 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     3481 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     3541 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     3601 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     3661 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     3721 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     3781 cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga
     3841 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     3901 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     3961 cagtgaattg taatacgact cactata
//
